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01-01-2013 | Original Paper

Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves

Authors: May-Hua Liao, Yuan-Nuan Tsai, Chi-Yu Yang, Chi-Long Juang, Ming-Yi Lee, Li-Hsueh Chang, Hsiao-Chuan Wen

Published in: Journal of Natural Medicines | Issue 1/2013

Abstract

Tagitinin C, a major sesquiterpenoid, was isolated from the leaves of Tithonia diversifolia. The high morbidity and mortality rate of hepatoma in Taiwan motivated our interest in the investigation of tagitinin C’s mechanism against the human hepatocellular carcinoma. The methanolic extract of leaves of T. diversifolia (TDM) and tagitinin C were found to have cytotoxic activities against human hepatoma Hep-G2 cells in the MTT assay with IC₅₀ values of 40.0 ± 2.0 and 2.0 ± 0.1 μg/mL, respectively. This compound induced population increase in the sub-G₁ phase and S phase arrest. Treatment with tagitinin C isolated from TDM resulted in activation of both caspase 3 and caspase 8 which suggested that the antiproliferative effect of this compound was caspase-dependent apoptosis. Magnetic resonance techniques indicated that the tumorigenisity of xenografts derived from Hep-G2 cells was retarded by the delivery of tagitinin C (15 μg/mouse/day) relative to the control counterparts.

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Title: Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves
Authors: May-Hua Liao
Yuan-Nuan Tsai
Chi-Yu Yang
Chi-Long Juang
Ming-Yi Lee
Li-Hsueh Chang
Hsiao-Chuan Wen
Publication date: 01-01-2013
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 1/2013
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-012-0652-0

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Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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