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Sport Sciences for Health
Published in: Sport Sciences for Health 3/2021

01-09-2021 | Insulins | Original Article

Protective effects of HIIT vs. CET exercise training on high-fat-high-fructose diet-induced hyperglycemia, hyperlipidemia, and histopathology of liver in rats: regulation of SIRT1/PGC-1α

Authors: Abolfazl Omidifar, Hossein Shirvani, Ramezan Ali Taheri, Sattar Gorgani-Firouzjae, Maryam Delfan, Fatemeh Kalaki-Jouybari, Soheyla Khakdan

Published in: Sport Sciences for Health | Issue 3/2021

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Abstract

Background

Disruption of the function of sirtuin1 (SIRT1) has been reported to link with diabetes and metabolic disorders.

Aim

The aim of this study was to investigate the effect of a modified high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) or continuous endurance training (CET) program on Sirt1 and PGC-1α mRNA gene expression in hepatocytes of high-fat/high-fructose diet-induced diabetes in rats.

Methods

Twenty-eight male Wistar rats (220 ± 20 g) were randomly divided into four groups of non-diabetic (NDC), diabetic control (received high fat high-fructose diet (HFHFD-DC)), CET (HFHFD-CET), and HIIT (HFHFD-HIIT). Diabetes was induced by feeding rats a high-fat (30%) high-fructose (20%) diet for 25 weeks. Following confirmed hyperglycemia, both HFHFD-CET (60–65% Vo2max) and HFHFD-HIIT (85–90% Vo2max) rats underwent treadmill exercise for 30 min, 5 days/week at 10 m/min for 8 weeks. Thereafter, blood and liver samples were obtained for biochemical studies. For histological analysis, the liver was fixed in 10% formalin, embedded in paraffin, and sectioned (5 μm thickness) for H&E staining. SIRT-1 and PGC-1α mRNA expression were measured by RT-PCR from the liver sample (frizzed sample). All data were presented as mean ± standard error mean and compared the differences by one-way analysis of variances. The alpha level was set at 0.05 (p < 0.05) for statistical difference.

Results

The results show that HIIT was more effective than CET to reduce vesicular steatosis. HIIT significantly enhanced the expression of SIRT-1 compared to the HFHFD-DC group (fold change: 1.47, p < 0.047). However, HIIT decreased PGC-1α expression compared to the HFHFD-DC group, but it was not significant (fold change: 1.32 and p < 0.247).

Conclusions

HIIT was more effective than CET in SIRT-1 expression augmentation in high-fat, high-fructose diabetic rats which suggests a possible treatment strategy in the context of type 2 diabetes.
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Metadata
Title
Protective effects of HIIT vs. CET exercise training on high-fat-high-fructose diet-induced hyperglycemia, hyperlipidemia, and histopathology of liver in rats: regulation of SIRT1/PGC-1α
Authors
Abolfazl Omidifar
Hossein Shirvani
Ramezan Ali Taheri
Sattar Gorgani-Firouzjae
Maryam Delfan
Fatemeh Kalaki-Jouybari
Soheyla Khakdan
Publication date
01-09-2021
Publisher
Springer Milan
Published in
Sport Sciences for Health / Issue 3/2021
Print ISSN: 1824-7490
Electronic ISSN: 1825-1234
DOI
https://doi.org/10.1007/s11332-021-00736-9

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