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01-09-2017 | Sleep Breathing Physiology and Disorders • Original Article

Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea

Authors: András Bikov, László Kunos, Éva Pállinger, Zsófia Lázár, Adrián Kis, Gábor Horváth, György Losonczy, Zsolt István Komlósi

Published in: Sleep and Breathing | Issue 3/2017

Abstract

Purpose

Microvesicles (MVs) have been implicated in the pathomechanism of obstructive sleep apnoea (OSA); however, the results are inconsistent, possibly due to an unrevealed temporal variation in circulating MV levels. We aimed to investigate the diurnal changes of MV fractions in OSA.

Methods

Peripheral blood was taken from 18 patients with OSA and 9 healthy subjects at different time points (11:00, 17:00, 21:00, 01:30 and 06:00). Samplings were repeated in nine OSA patients after 2 months of continuous positive airway pressure (CPAP) therapy. CD41+, CD62P+, glycophorin A+ and Annexin V+ MVs were determined with flow cytometry. Areas under the MV concentrations-time curves (AUC) were calculated and correlated with the severity of OSA.

Results

A significant diurnal variability of plasma CD41+ and Annexin V+ MVs was observed only in OSA with a marked peak at 17:00. There was a direct correlation between CD41+ MV AUCs and the severity of OSA. CPAP treatment reduced diurnal variability in both CD41+ and Annexin V+ MV levels.

Conclusions

The relationship between the diurnal variability of CD41+ MVs and disease severity as well as the effect of CPAP treatment on MV levels support the role of MVs in the pathophysiology of OSA. More importantly, considering the significant diurnal variation in circulating MV levels, introduction of strict protocols for blood sampling is required for MV measurements.

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Title: Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea
Authors: András Bikov
László Kunos
Éva Pállinger
Zsófia Lázár
Adrián Kis
Gábor Horváth
György Losonczy
Zsolt István Komlósi
Publication date: 01-09-2017
Publisher: Springer International Publishing
Published in: Sleep and Breathing / Issue 3/2017
Print ISSN: 1520-9512
Electronic ISSN: 1522-1709
DOI: https://doi.org/10.1007/s11325-017-1464-y

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