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01-04-2017 | Research Article

Targeted Superparamagnetic Iron Oxide Nanoparticles for In Vivo Magnetic Resonance Imaging of T-Cells in Rheumatoid Arthritis

Authors: Chih-Lung Chen, Tiing Yee Siow, Cheng-Hung Chou, Chen-Hsuan Lin, Ming-Huang Lin, Yung-Chu Chen, Wen-Yuan Hsieh, Shian-Jy Wang, Chen Chang

Published in: Molecular Imaging and Biology | Issue 2/2017

Abstract

Purpose

The purpose of the study is to develop a targeted nanoparticle platform for T cell labeling and tracking in vivo.

Procedures

Through carboxylation of the polyethylene glycol (PEG) surface of SPION, carboxylated-PEG-SPION (IOPC) was generated as a precursor for further conjugation with the targeting probe. The IOPC could readily cross-link with a variety of amide-containing molecules by exploiting the reaction between 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide and N-hydroxysuccinimide. The subsequent conjugation of monoclonal anti-CD3 antibody with IOPC made it possible to construct a magnetic resonance imaging (MRI) contrast agente (CA) that targets T cells, named IOPC-CD3.

Results

IOPC-CD3 was found to have high transverse relaxivity, good targeting selectivity, and good safety profile in vitro. The utility of this newly synthesized CA was explored in an in vivo rodent collagen-induced arthritis (CIA) model of rheumatoid arthritis. Serial MRI experiments revealed a selective decrease in the signal-to-noise ratio of the femoral growth plates of CIA rats infused with IOPC-CD3, with this finding being consistent with immunohistochemical results showing the accumulation of T cells and iron oxide nanoparticles in the corresponding region.

Conclusions

Together with the abovementioned desirable features, these results indicate that IOPC-CD3 offers a promising prospect for a wide range of cellular and molecular MRI applications.

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Title: Targeted Superparamagnetic Iron Oxide Nanoparticles for In Vivo Magnetic Resonance Imaging of T-Cells in Rheumatoid Arthritis
Authors: Chih-Lung Chen
Tiing Yee Siow
Cheng-Hung Chou
Chen-Hsuan Lin
Ming-Huang Lin
Yung-Chu Chen
Wen-Yuan Hsieh
Shian-Jy Wang
Chen Chang
Publication date: 01-04-2017
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 2/2017
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-016-1001-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Targeted Superparamagnetic Iron Oxide Nanoparticles for In Vivo Magnetic Resonance Imaging of T-Cells in Rheumatoid Arthritis

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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