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01-12-2014 | Research Article

Accumulation of Trans-1-Amino-3-[¹⁸F]Fluorocyclobutanecarboxylic Acid in Prostate Cancer due to Androgen-Induced Expression of Amino Acid Transporters

Authors: Hiroyuki Okudaira, Shuntaro Oka, Masahiro Ono, Takeo Nakanishi, David M. Schuster, Masato Kobayashi, Mark M. Goodman, Ikumi Tamai, Keiichi Kawai, Yoshifumi Shirakami

Published in: Molecular Imaging and Biology | Issue 6/2014

Abstract

Purpose

Androgens play a crucial role in prostate cancer progression, and trans-1-amino-3-[¹⁸F]fluorocyclobutanecarboxylic acid (anti-[¹⁸ F]FACBC) are used for visualization of prostate cancer. We examined the effect of androgen on the expression of amino acid transporters related to anti-[¹⁸F]FACBC transport and uptake of trans-1-amino-3-fluoro-[1-¹⁴C]cyclobutanecarboxylic acid (anti-[¹⁴C]FACBC).

Procedures

Expression of amino acid transporters and uptake of anti-[¹⁴C]FACBC in androgen receptor (AR)-positive LNCaP and AR-negative DU145 human prostate cancer cells cultured with/without 5α-dihydrotestosterone (DHT) and the effect of bicalutamide, an AR antagonist, on DHT-associated changes were investigated.

Results

DHT stimulated the expression of amino acid transporters ASCT2, SNAT5, 4F2 heavy chain, and LAT3 in LNCaP but not in DU145 cells. Anti-[¹⁴C]FACBC uptake was enhanced, in a DHT-dependent manner, in LNCaP cells only.

Conclusions

DHT enhanced the expression of ASCT2, the transporter responsible for anti-[¹⁸F]FACBC uptake, thereby increasing anti-[¹⁴C]FACBC uptake in AR-positive LNCaP cells. Androgen-mediated induction may contribute to the distinct anti-[¹⁸F]FACBC accumulation pattern in prostate cancer.

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Title: Accumulation of Trans-1-Amino-3-[18F]Fluorocyclobutanecarboxylic Acid in Prostate Cancer due to Androgen-Induced Expression of Amino Acid Transporters
Authors: Hiroyuki Okudaira
Shuntaro Oka
Masahiro Ono
Takeo Nakanishi
David M. Schuster
Masato Kobayashi
Mark M. Goodman
Ikumi Tamai
Keiichi Kawai
Yoshifumi Shirakami
Publication date: 01-12-2014
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 6/2014
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-014-0756-x

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Accumulation of Trans-1-Amino-3-[¹⁸F]Fluorocyclobutanecarboxylic Acid in Prostate Cancer due to Androgen-Induced Expression of Amino Acid Transporters

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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