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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 1/2014

Open Access 01-02-2014 | Research Article

68Ga-Triacetylfusarinine C and 68Ga-Ferrioxamine E for Aspergillus Infection Imaging: Uptake Specificity in Various Microorganisms

Authors: Milos Petrik, Hubertus Haas, Peter Laverman, Markus Schrettl, Gerben M. Franssen, Michael Blatzer, Clemens Decristoforo

Published in: Molecular Imaging and Biology | Issue 1/2014

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Abstract

Purpose

68Ga-triacetylfusarinine C (68Ga-TAFC) and 68Ga-ferrioxamine E (68Ga-FOXE) showed excellent targeting properties in Aspergillus fumigatus rat infection model. Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).

Procedures

The in vitro uptake of 68Ga-TAFC and 68Ga-FOXE was studied in various fungal, bacterial and yeast cultures as well as in H1299 cells. The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models.

Results

68Ga-TAFC and 68Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for 68Ga-FOXE in Staphylococcus aureus. 68Ga-TAFC and 68Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.

Conclusions

We have shown that 68Ga-FOXE and 68Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. 68Ga-TAFC showed higher specificity, while 68Ga-FOXE showed higher sensitivity.
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Metadata
Title
68Ga-Triacetylfusarinine C and 68Ga-Ferrioxamine E for Aspergillus Infection Imaging: Uptake Specificity in Various Microorganisms
Authors
Milos Petrik
Hubertus Haas
Peter Laverman
Markus Schrettl
Gerben M. Franssen
Michael Blatzer
Clemens Decristoforo
Publication date
01-02-2014
Publisher
Springer US
Published in
Molecular Imaging and Biology / Issue 1/2014
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-013-0654-7

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