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01-12-2011 | Research Article

Impact of Indium-111 Oxine Labelling on Viability of Human Mesenchymal Stem Cells In Vitro, and 3D Cell-Tracking Using SPECT/CT In Vivo

Authors: Franz Josef Gildehaus, Florian Haasters, Inga Drosse, Erika Wagner, Christian Zach, Wolf Mutschler, Paul Cumming, Peter Bartenstein, Matthias Schieker

Published in: Molecular Imaging and Biology | Issue 6/2011

Abstract

Purpose

This study investigates the effects of ¹¹¹In-oxine incorporation on human mesenchymal stem cells’ (hMSC) biology and viability, and the applicability of ¹¹¹In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.

Procedures

HMSC were labelled with 10 Bq/cell. Cellular retention of radioactivity, cell survival, and migration were evaluated over 48 h. Metabolic activity was assessed over 14 days and the hMSC’s stem cell character was evaluated. Serial SPECT/CT was performed after intra-osseous injection to athymic rats over 48 h.

Results

Labelling efficiency was 25%, with 61% of incorporated ¹¹¹In remaining in the hMSC at 48 h. The radiolabelling was without effect on cell viability, stem cell character, and plasticity, whereas metabolic activity and migration were significantly reduced. Grafted cells could be imaged in situ with SPECT/CT.

Conclusions

¹¹¹In-oxine labelling moderately impaired hMSC’s functional integrity while preserving their stem cell character. Combined SPECT/CT imaging of ¹¹¹In-oxine-labelled hMSC opens the possibility for non-invasive sequential monitoring of therapeutic stem cells.

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Title: Impact of Indium-111 Oxine Labelling on Viability of Human Mesenchymal Stem Cells In Vitro, and 3D Cell-Tracking Using SPECT/CT In Vivo
Authors: Franz Josef Gildehaus
Florian Haasters
Inga Drosse
Erika Wagner
Christian Zach
Wolf Mutschler
Paul Cumming
Peter Bartenstein
Matthias Schieker
Publication date: 01-12-2011
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 6/2011
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-010-0439-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Impact of Indium-111 Oxine Labelling on Viability of Human Mesenchymal Stem Cells In Vitro, and 3D Cell-Tracking Using SPECT/CT In Vivo

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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