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Open Access 01-10-2011 | Research Article

Intraoperative Multispectral Fluorescence Imaging for the Detection of the Sentinel Lymph Node in Cervical Cancer: A Novel Concept

Authors: Lucia M. A. Crane, George Themelis, Rick G. Pleijhuis, Niels J. Harlaar, Athanasios Sarantopoulos, Henriette J. G. Arts, Ate G. J. van der Zee, Ntziachristos Vasilis, Gooitzen M. van Dam

Published in: Molecular Imaging and Biology | Issue 5/2011

Abstract

Purpose

Real-time intraoperative near-infrared fluorescence (NIRF) imaging is a promising technique for lymphatic mapping and sentinel lymph node (SLN) detection. The purpose of this technical feasibility pilot study was to evaluate the applicability of NIRF imaging with indocyanin green (ICG) for the detection of the SLN in cervical cancer.

Procedures

In ten patients with early stage cervical cancer, a mixture of patent blue and ICG was injected into the cervix uteri during surgery. Real-time color and fluorescence videos and images were acquired using a custom-made multispectral fluorescence camera system.

Results

Real-time fluorescence lymphatic mapping was observed in vivo in six patients; a total of nine SLNs were detected, of which one (11%) contained metastases. Ex vivo fluorescence imaging revealed the remaining fluorescent signal in 11 of 197 non-sentinel LNs (5%), of which one contained metastatic tumor tissue. None of the non-fluorescent LNs contained metastases.

Conclusions

We conclude that lymphatic mapping and detection of the SLN in cervical cancer using intraoperative NIRF imaging is technically feasible. However, the technique needs to be refined for full applicability in cervical cancer in terms of sensitivity and specificity.

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Title: Intraoperative Multispectral Fluorescence Imaging for the Detection of the Sentinel Lymph Node in Cervical Cancer: A Novel Concept
Authors: Lucia M. A. Crane
George Themelis
Rick G. Pleijhuis
Niels J. Harlaar
Athanasios Sarantopoulos
Henriette J. G. Arts
Ate G. J. van der Zee
Ntziachristos Vasilis
Gooitzen M. van Dam
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 5/2011
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-010-0425-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Intraoperative Multispectral Fluorescence Imaging for the Detection of the Sentinel Lymph Node in Cervical Cancer: A Novel Concept

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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