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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 3/2007

01-05-2007 | Research Article

A Small Animal Positron Emission Tomography Study of the Effect of Chemotherapy and Hormonal Therapy on the Uptake of 2-Deoxy-2-[F-18]fluoro-d-glucose in Murine Models of Breast Cancer

Authors: Antonio Aliaga, Jacques A. Rousseau, Jules Cadorette, Étienne Croteau, Johan E. van Lier, Roger Lecomte, François Bénard

Published in: Molecular Imaging and Biology | Issue 3/2007

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Abstract

Purpose

We used small animal positron emission tomography (PET) imaging to monitor the time-course of tumor metabolic response to hormone and chemotherapy in a murine model of hormone-sensitive breast cancer.

Procedures

Estrogen receptor positive murine mammary carcinomas were inoculated in Balb/c mice. Small animal PET imaging using 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) was used to assess tumor metabolic activity. Imaging was done before and at days 1, 7, and 14 after the administration of doxorubicin, methotrexate, letrozole, or placebo. The tumor uptake of FDG was calculated from a region-of-interest drawn around the tumor.

Results

All treatments resulted in a decrease in tumor growth rate and end volume compared to untreated control. FDG uptake was also markedly decreased after treatment although a flare reaction was observed on PET at day 7, the intensity of which varied according to the treatment modality.

Conclusion

PET imaging is sensitive to detect early changes associated with therapy in murine breast cancer models. A flare reaction was observed 7 days after the initiation of therapy.
Literature
1.
Volk EL, Rohde K, Rhee M, et al. (2000) Methotrexate cross-resistance in a mitoxantrone-selected multidrug-resistant MCF7 breast cancer cell line is attributable to enhanced energy-dependent drug efflux. Cancer Res 60:3514–3521PubMed
2.
Dowsett M (2001) Overexpression of HER-2 as a resistance mechanism to hormonal therapy for breast cancer. Endocr-Relat Cancer 8:191–519PubMedCrossRef
3.
Clarke R, Leonessa F, Welch JN, Skaar C (2001) Cellular and molecular pharmacology of antiestrogen action and resistance. Pharmacol Rev 53:25–71PubMed
4.
Benard F, Turcotte E (2005) Imaging in breast cancer: single-photon computed tomography and positron-emission tomography. Breast Cancer Res 7:153–162PubMedCrossRef
5.
Aliaga A, Rousseau JA, Ouellette R, et al. (2004) Breast cancer models to study the expression of estrogen receptors with small animal PET imaging. Nucl Med Biol 31:761–770PubMedCrossRef
6.
Hamacher K, Coenen HH, Stocklin G (1986) Efficient stereospecific synthesis of no-carrier-added 2-[18F]-fluoro-2-deoxy-D-glucose using aminopolyether supported nucleophilic substitution. J Nucl Med 27:235–238PubMed
7.
Lanari C, Luthy I., Lamb CA, et al. (2001) Five novel hormone-responsive cell lines derived from murine mammary ductal carcinomas: in vivo and in vitro effects of estrogens and progestins. Cancer Res 61:293–302PubMed
8.
Tomayko MM, Reynolds CP (1989) Determination of subcutaneous tumor size in athymic (nude) mice. Cancer Chemother Pharmacol 24:148–154PubMedCrossRef
9.
Lecomte R, Cadorette J, Richard P, Rodrigue S, Rouleau D (1994) Design and engineering aspects of a high resolution positron tomograph for small animal imaging. IEEE Trans Nucl Sci 41:1446–1452CrossRef
10.
Lecomte R, Cadorette J, Rodrigue S, et al. (1996) Initial results from the Sherbrooke avalanche photodiode positron tomograph. IEEE Trans Nucl Sci 43:1952–1957CrossRef
11.
Selivanov V, Picard Y, Cadorette J, Rodrigue S, Lecomte R (2000) Detector response models for statistical iterative image reconstruction in high resolution PET. IEEE Trans Nucl Sci 47:1168–1175CrossRef
12.
Keyes JW Jr (1995) SUV: standard uptake or silly useless value? J Nucl Med 36:1836–1839PubMed
13.
Spaepen K, Stroobants S, Dupont P, et al. (2003) [(18)F]FDG PET monitoring of tumour response to chemotherapy: does [(18)F]FDG uptake correlate with the viable tumour cell fraction? Eur J Nucl Med 30:682–688CrossRef
14.
Yoshioka T, Takahashi H, Oikawa H, et al. (1997) Influence of chemotherapy on FDG uptake by human cancer xenografts in nude mice. J Nucl Med 38:714–717PubMed
15.
Mortimer JE, Dehdashti F, Siegel BA, et al. (2001) Metabolic flare: indicator of hormone responsiveness in advanced breast cancer. J Clin Oncol 19:2797–2803PubMed
16.
Plotkin D, Lechner JJ, Jung WE, Rosen PJ (1978) Tamoxifen flare in advanced breast cancer. JAMA 240:2644–2646PubMedCrossRef
17.
Zigrossi P, Brustia M, Bobbio F, Campanini M (2001) Flare and tumor lysis syndrome with atypical features after letrozole therapy in advanced breast cancer. A case report. Ann Ital Med Int 16:112–117PubMed
18.
Greenwald ES (1983) Megestrol acetate flare. Cancer Treat Rep 67:405PubMed
Metadata
Title
A Small Animal Positron Emission Tomography Study of the Effect of Chemotherapy and Hormonal Therapy on the Uptake of 2-Deoxy-2-[F-18]fluoro-d-glucose in Murine Models of Breast Cancer
Authors
Antonio Aliaga
Jacques A. Rousseau
Jules Cadorette
Étienne Croteau
Johan E. van Lier
Roger Lecomte
François Bénard
Publication date
01-05-2007
Publisher
Springer-Verlag
Published in
Molecular Imaging and Biology / Issue 3/2007
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-007-0091-6

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