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Molecular Imaging and Biology

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01-07-2006 | Research Article

Near-Infrared Fluorescence Imaging of Tumor Integrin α_vβ₃ Expression with Cy7-Labeled RGD Multimers

Authors: Yun Wu, Weibo Cai, Xiaoyuan Chen

Published in: Molecular Imaging and Biology | Issue 4/2006

Abstract

Purpose

Cell adhesion molecule integrin α_vβ₃ is an excellent target for tumor interventions because of its unique expression on the surface of several types of solid tumor cells and on almost all sprouting tumor vasculatures. Here, we describe the development of near-infrared (NIR) fluorochrome Cy7-labeled RGD peptides for tumor integrin targeting.

Procedures

Mono-, di-, and tetrameric RGD peptides were synthesized and conjugated with Cy7. The integrin specificity of these fluorescent probes was tested in vitro for receptor binding assay and fluorescence microscopy and in vivo for subcutaneous U87MG tumor targeting.

Results

The tetrameric RGD peptide probe with the highest integrin affinity showed the highest tumor activity accumulation and strongest tumor-to-normal tissue contrast. This uptake is integrin-specific as the signal accumulated in the tumor can be effectively blocked by unconjugated RGD peptide antagonist of integrin α_vβ₃.

Conclusions

Noninvasive NIR fluorescence imaging is able to detect and semiquantify tumor integrin expression based upon the highly potent tetrameric RGD peptide probe.

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Title: Near-Infrared Fluorescence Imaging of Tumor Integrin αvβ3 Expression with Cy7-Labeled RGD Multimers
Authors: Yun Wu
Weibo Cai
Xiaoyuan Chen
Publication date: 01-07-2006
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 4/2006
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-006-0041-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Near-Infrared Fluorescence Imaging of Tumor Integrin α_vβ₃ Expression with Cy7-Labeled RGD Multimers

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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