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Open Access 01-02-2012 | Urology – Original Paper

Changes in interstitial cell of Cajal-like cells density in congenital ureteropelvic junction obstruction

Authors: Piotr Koleda, Wojciech Apoznanski, Zdzislaw Wozniak, Leslaw Rusiecki, Tomasz Szydelko, Witold Pilecki, Marcin Polok, Dariusz Kalka, Artur Pupka

Published in: International Urology and Nephrology | Issue 1/2012

Abstract

Purpose

The authors examined the number of interstitial cells of Cajal-like cells (ICC-LCs) in obstructed ureteropelvic junction (UPJ) in comparison with normal UPJ specimens and age-related changes.

Materials and methods

A total of 20 human formalin fixed, paraffin-embedded specimens of intrinsic UPJO from children at the mean age of 8.1 years (age range: 8 months–16.8 years) and 5 control samples from children at the mean age of 2.3 years (range 2.4 months–7.4 years) were investigated immunohistochemically for the expression of c-kit proto-oncogene by light microscopy with computerized image analysis. The results were examined by Fisher’s exact test, Yates’ chi-square test, and t test for Pearson’s correlation coefficient. A P value less than 0.05 was considered statistically significant.

Results

The number of ICC-LCs-dense fields was significantly higher in UPJO specimens in comparison with the normal group (P = 0.0004). The number of ICC-LCs-sparse fields was significantly lowered in UPJO specimens in comparison with the normal group (P = 0.0122). There was no significant difference in the number of ICC-LCs-medium fields in obstructed and normal UPJ specimens. The number of ICC-LCs was decreasing significantly with increasing age of the patients with UPJO (P = 0.0038).

Conclusions

The higher density of c-kit-positive ICC-LCs in UPJ may manifest the compensation of altered peristalsis in UPJO. The number of ICC-LCs-dense fields decreasing with age may show the failure of compensation and regression of the compensatory changes.

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Title: Changes in interstitial cell of Cajal-like cells density in congenital ureteropelvic junction obstruction
Authors: Piotr Koleda
Wojciech Apoznanski
Zdzislaw Wozniak
Leslaw Rusiecki
Tomasz Szydelko
Witold Pilecki
Marcin Polok
Dariusz Kalka
Artur Pupka
Publication date: 01-02-2012
Publisher: Springer Netherlands
Published in: International Urology and Nephrology / Issue 1/2012
Print ISSN: 0301-1623
Electronic ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-011-9970-5

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