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01-05-2013 | Laboratory Investigation

Comparison of microRNA expression levels between initial and recurrent glioblastoma specimens

Authors: Aysegül Ilhan-Mutlu, Adelheid Wöhrer, Anna Sophie Berghoff, Georg Widhalm, Christine Marosi, Ludwig Wagner, Matthias Preusser

Published in: Journal of Neuro-Oncology | Issue 3/2013

Abstract

Glioblastoma is the most frequent primary brain tumour in adults. Recent therapeutic advances increased patient's survival, but tumour recurrence inevitably occurs. The pathobiological mechanisms involved in glioblastoma recurrence are still unclear. MicroRNAs are small RNAs proposed o have important roles for cancer including proliferation, aggressiveness and metastases development. There exist only few data on the involvement of microRNAs in glioblastoma recurrence. We selected the following 7 microRNAs with potential relevance for glioblastoma pathobiology by means of a comprehensive literature search: microRNA-10b, microRNA-21, microRNA-181b, microRNA-181c, microRNA-195, microRNA-221 and microRNA-222. We further selected 15 primary glioblastoma patients, of whom formalin fixed and paraffin embedded tissue (FFPE) of the initial and recurrence surgery were available. All patients had received first line treatment consisting of postoperative combined radiochemotherapy with temozolomide (n = 15). Non-neoplastic brain tissue samples from 3 patients with temporal lobe epilepsy served as control. The expression of the microRNAs were analysed by RT-qPCR. These were correlated with each other and with clinical parameters. All microRNAs showed detectable levels of expressions in glioblastoma group, whereas microRNA-10b was not detectable in epilepsy patients. MicroRNAs except microRNA-21 showed significantly higher levels in epilepsy patients when compared to the levels of first resection of glioblastoma. Comparison of microRNA levels between first and second resections revealed no significant change. Cox regression analyses showed no significant association of microRNA expression levels in the tumor tissue with progression free survival times. Expression levels of microRNA-10b, microRNA-21, microRNA-181b, microRNA-181c, microRNA-195, microRNA-221 and microRNA-222 do not differ significantly between initial and recurrent glioblastoma.

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Title: Comparison of microRNA expression levels between initial and recurrent glioblastoma specimens
Authors: Aysegül Ilhan-Mutlu
Adelheid Wöhrer
Anna Sophie Berghoff
Georg Widhalm
Christine Marosi
Ludwig Wagner
Matthias Preusser
Publication date: 01-05-2013
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 3/2013
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-013-1078-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Comparison of microRNA expression levels between initial and recurrent glioblastoma specimens

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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