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01-08-2012 | Laboratory Investigation

Triptolide inhibits proliferation and invasion of malignant glioma cells

Authors: Haipeng Zhang, Wenbo Zhu, Xingwen Su, Sihan Wu, Yuan Lin, Jingjie Li, Youqiong Wang, Jingkao Chen, Yuxi Zhou, Pengxin Qiu, Guangmei Yan, Shujin Zhao, Jun Hu, Jingxia Zhang

Published in: Journal of Neuro-Oncology | Issue 1/2012

Abstract

Malignant glioma is the most devastating and aggressive tumor in brain, characterized by rapid proliferation and diffuse invasion. Chemotherapy and radiotherapy are the pivotal strategies after surgery; however, high drug resistance of malignant glioma and the blood–brain barrier usually render chemotherapy drugs ineffective. Here, we find that triptolide, a small molecule with high lipid solubility, is capable of inhibiting proliferation and invasion of malignant glioma cells effectively. In both investigated malignant glioma cell lines, triptolide repressed cell proliferation via inducing cell cycle arrest in G0/G1 phase, associated with downregulation of G0/G1 cell cycle regulators cyclin D1, CDK4, and CDK6 followed by reduced phosphorylation of retinoblastoma protein (Rb). In addition, triptolide induced morphological change of C6 cells through downregulation of protein expression of MAP-2 and inhibition of activities of GTPases Cdc42 and Rac1/2/3, thus significantly suppressing migratory and invasive capacity. Moreover, in an in vivo tumor model, triptolide delayed growth of malignant glioma xenografts. These findings suggest an important inhibitory action of triptolide on proliferation and invasion of malignant glioma, and encourage triptolide as a candidate for glioma therapy.

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Title: Triptolide inhibits proliferation and invasion of malignant glioma cells
Authors: Haipeng Zhang
Wenbo Zhu
Xingwen Su
Sihan Wu
Yuan Lin
Jingjie Li
Youqiong Wang
Jingkao Chen
Yuxi Zhou
Pengxin Qiu
Guangmei Yan
Shujin Zhao
Jun Hu
Jingxia Zhang
Publication date: 01-08-2012
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 1/2012
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-012-0885-5

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Triptolide inhibits proliferation and invasion of malignant glioma cells

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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