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01-03-2012 | Laboratory Investigation - Human/Animal Tissue

Evaluation of radiation necrosis and malignant glioma in rat models using diffusion tensor MR imaging

Authors: Silun Wang, Yifei Chen, Bachchu Lal, Eric Ford, Erik Tryggestad, Michael Armour, Kun Yan, John Laterra, Jinyuan Zhou

Published in: Journal of Neuro-Oncology | Issue 1/2012

Abstract

Standard MRI cannot distinguish between radiation necrosis and tumor progression; however, this distinction is critical in the assessment of tumor response to therapy. In this study, one delayed radiation necrosis model (dose, 40 Gy; radiation field, 10 × 10 mm²; n = 13) and two orthotopic glioma models in rats (9L gliosarcoma, n =8; human glioma xenografts, n = 5) were compared using multiple diffusion tensor imaging (DTI) indices. A visible isotropic apparent diffusion coefficient (ADC) pattern was observed in the lesion due to radiation necrosis, which consisted of a hypointense central zone and a hyperintense peripheral zone. There were significantly lower ADC, parallel diffusivity, and perpendicular diffusivity in the necrotic central zone than in the peripheral zone (all P < 0.001). When radiation-induced necrosis was compared with viable tumor, radiation necrosis had significantly lower ADC than 9L gliosarcoma and human glioma xenografts (both P < 0.01) in the central zone, and significantly lower fractional anisotropy than 9L gliosarcoma (P = 0.005) and human glioma xenografts (P = 0.012) in the peripheral zone. Histological analysis revealed parenchymal coagulative necrosis in the central zone, and damaged vessels and reactive astrogliosis in the peripheral zone. These data suggest that qualitative and quantitative analysis of the DTI maps can provide useful information by which to distinguish between radiation necrosis and viable glioma.

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Title: Evaluation of radiation necrosis and malignant glioma in rat models using diffusion tensor MR imaging
Authors: Silun Wang
Yifei Chen
Bachchu Lal
Eric Ford
Erik Tryggestad
Michael Armour
Kun Yan
John Laterra
Jinyuan Zhou
Publication date: 01-03-2012
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 1/2012
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-011-0719-x

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Evaluation of radiation necrosis and malignant glioma in rat models using diffusion tensor MR imaging

Abstract

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