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01-07-2009 | Laboratory Investigation - Human/Animal Tissue

miR-21 and 221 upregulation and miR-181b downregulation in human grade II–IV astrocytic tumors

Authors: Alfredo Conti, M’Hammed Aguennouz, Domenico La Torre, Chiara Tomasello, Salvatore Cardali, Filippo F. Angileri, Francesca Maio, Annamaria Cama, Antonino Germanò, Giuseppe Vita, Francesco Tomasello

Published in: Journal of Neuro-Oncology | Issue 3/2009

Abstract

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. Recent evidence indicates that miRNAs can function both as tumor suppressors and as oncogenes. It has been demonstrated that in glioblastoma multiforme miR-21 and 221 are upregulated whereas miR-128 and 181 are downregulated. Expression of miR-21, 221, 128a, 128b, 128c, 181a, 181b, 181c was studied using real-time quantitative reverse transcriptase polymerase chain reaction and northern blotting for human astrocytic tumors with different grade of malignancy. miR-21 and 221 were overexpressed in glioma samples, whereas miRNA 181b was downregulated compared with normal brain tissue. miRNA-21 was hyperexpressed in all tumor samples whereas higher levels of miRNA-221 were found in high-grade gliomas. This study is the first analysis of miRNAs in astrocytic tumor at different stages of malignancy. The different expression pattern observed in tumors at different stages of malignancy is probably dependent on the cell-specific repertoire of target genes of tumors sharing different molecular pathways activity and suggests miRNAs may have also a place in diagnosis and staging of brain tumors.

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Title: miR-21 and 221 upregulation and miR-181b downregulation in human grade II–IV astrocytic tumors
Authors: Alfredo Conti
M’Hammed Aguennouz
Domenico La Torre
Chiara Tomasello
Salvatore Cardali
Filippo F. Angileri
Francesca Maio
Annamaria Cama
Antonino Germanò
Giuseppe Vita
Francesco Tomasello
Publication date: 01-07-2009
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 3/2009
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-009-9797-4

2024 ASCO Annual Meeting

Springer Medicine

miR-21 and 221 upregulation and miR-181b downregulation in human grade II–IV astrocytic tumors

Abstract

2024 ASCO Annual Meeting

Springer Medicine

Abstract

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