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30-06-2023 | Glioblastoma | Original Article

Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme

Authors: Renjie Wang, Qi Li, Xiaolei Chu, Nan Li, Haiqian Liang, Feng He

Published in: Metabolic Brain Disease | Issue 7/2023

Abstract

Evidence suggests that non-coding RNAs have a role in glioblastoma multiforme (GBM), although the regulatory mechanisms controlled by competing endogenous RNAs (ceRNAs) in GBM are still poorly understood and infrequently described. This research extensively analyzed circRNA, lncRNA, miRNA, and mRNA expression changes in GBM patients. RNA-sequencing analyses were conducted to investigate differentially expressed genes (DEGs), lncRNAs (DELs), miRNAs (DEMs), and circRNAs (DECs) in the GBM. In this study, researchers found that GBM patients and healthy controls differed in the presence of 1224 DECs, 1406 DELs, 229 DEMs, and 2740 DEGs. PPI network analysis demonstrated that CEACAM5, CXCL17, FAM83A, TMPRSS4, and GGPRC5A were hub genes and enriched in modules. Then a ceRNA network was constructed with 8 circRNA, 7 lncRNAs, 16 miRNAs, and 17 mRNAs. Overall, the ceRNA interaction axes that were found may prove to be pivotal therapeutic targets for treating GBM.

Abdul-Wahid A, Cydzik M, Fischer NW, Prodeus A, Shively JE, Martel A et al (2018) Serum-derived carcinoembryonic antigen (CEA) activates fibroblasts to induce a local re-modeling of the extracellular matrix that favors the engraftment of CEA-expressing tumor cells. Int J Cancer 143:1963–1977CrossRefPubMedPubMedCentral

Ambrosi C, Scribano D, Sarshar M, Zagaglia C, Singer BB, Palamara AT (2020) Acinetobacter baumannii targets Human Carcinoembryonic Antigen-Related cell adhesion molecules (CEACAMs) for Invasion of Pneumocytes. mSystems. ;5

Azari F, Kennedy GT, Chang A, Bernstein E, Nadeem B, Pèlegrin A et al (2022) Glycoprotein receptor CEACAM5-Targeted intraoperative Molecular Imaging Tracer in Non-Small Cell Lung Cancer. The Annals of thoracic surgery

Baek DS, Kim YJ, Vergara S, Conard A, Adams C, Calero G et al (2022) A highly-specific fully-human antibody and CAR-T cells targeting CD66e/CEACAM5 are cytotoxic for CD66e-expressing cancer cells in vitro and in vivo. Cancer Lett 525:97–107CrossRefPubMed

Basso J, Paggi MG, Fortuna A, Vitorino C (2022) Deciphering specific miRNAs in brain tumors: a 5-miRNA signature in glioblastoma. 297:507–521

Cheng J, Meng J, Zhu L, Peng Y (2020) Exosomal non-coding RNAs in glioma: biological functions and potential clinical applications. Mol Cancer 19:66CrossRefPubMedPubMedCentral

Choreño-Parra JA, Thirunavukkarasu S, Zúñiga J, Khader SA (2020) The protective and pathogenic roles of CXCL17 in human health and disease: potential in respiratory medicine. Cytokine Growth Factor Rev 53:53–62CrossRefPubMedPubMedCentral

Gisina A, Novikova S, Kim Y, Sidorov D, Bykasov S, Volchenko N et al (2021) CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells. Cancer Biomark A 32:85–98CrossRef

Guo X, Piao H (2021) Research Progress of circRNAs in Glioblastoma. Front cell Dev biology 9:791892CrossRef

Holmer R, Wätzig GH, Tiwari S, Rose-John S, Kalthoff H (2015) Interleukin-6 trans-signaling increases the expression of carcinoembryonic antigen-related cell adhesion molecules 5 and 6 in colorectal cancer cells. BMC Cancer 15:975CrossRefPubMedPubMedCentral

Ji H, Song H, Wang Z, Jiao P, Xu J, Li X et al (2021) FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma. 19:423

Jia B, Liu W, Gu J, Wang J, Lv W, Zhang W et al (2019) MiR-7-5p suppresses stemness and enhances temozolomide sensitivity of drug-resistant glioblastoma cells by targeting Yin Yang 1. Exp Cell Res 375:73–81CrossRefPubMed

Kelleher M, Singh R, O’Driscoll CM, Melgar S (2019) Carcinoembryonic antigen (CEACAM) family members and inflammatory bowel disease. Cytokine Growth Factor Rev 47:21–31CrossRefPubMed

Le Rhun E, Preusser M, Roth P, Reardon DA, van den Bent M, Wen P et al (2019) Molecular targeted therapy of glioblastoma. Cancer Treat Rev 80:101896CrossRefPubMed

Li L, Ji Y, Chen Y, Zhen Z (2021) MiR-325-3p mediate the CXCL17/CXCR8 axis to regulate angiogenesis in hepatocellular carcinoma. Cytokine 141:155436CrossRefPubMed

Liu R, Dai W (2021) CircCDC45 promotes the malignant progression of glioblastoma by modulating the miR-485-5p/CSF-1 axis. 21:1090

Ma R, Taphoorn MJB, Plaha P (2021) Advances in the management of glioblastoma. J Neurol Neurosurg Psychiatry 92:1103–1111CrossRefPubMed

Ohba S, Hirose Y (2016) Current and future drug treatments for glioblastomas. Curr Med Chem 23:4309–4316CrossRefPubMed

Ohgaki H, Kleihues P (2013) The definition of primary and secondary glioblastoma. Clin cancer research: official J Am Association Cancer Res 19:764–772CrossRef

Omuro A, DeAngelis LM (2013) Glioblastoma and other malignant gliomas: a clinical review. JAMA 310:1842–1850CrossRefPubMed

Peng Z, Liu C, Wu M (2018) New insights into long non-coding RNAs and their roles in glioma. Mol Cancer 17:61CrossRefPubMedPubMedCentral

Powell E, Shao J, Picon HM, Bristow C, Ge Z, Peoples M et al (2018) A functional genomic screen in vivo identifies CEACAM5 as a clinically relevant driver of breast cancer metastasis. NPJ breast cancer 4:9CrossRefPubMedPubMedCentral

Robin AM, Lee I, Kalkanis SN (2017) Reoperation for recurrent Glioblastoma Multiforme. Neurosurg Clin North Am 28:407–428CrossRef

Shea A, Harish V, Afzal Z, Chijioke J, Kedir H, Dusmatova S et al (2016) MicroRNAs in glioblastoma multiforme pathogenesis and therapeutics. Cancer Med 5:1917–1946CrossRefPubMedPubMedCentral

Simpson L, Galanis E (2006) Recurrent glioblastoma multiforme: advances in treatment and promising drug candidates. Expert Rev Anticancer Ther 6:1593–1607CrossRefPubMed

Wang Y, Xu R, Zhang D, Lu T, Yu W, Wo Y et al (2019) Circ-ZKSCAN1 regulates FAM83A expression and inactivates MAPK signaling by targeting mir-330-5p to promote non-small cell lung cancer progression. Translational lung cancer research 8:862–875CrossRefPubMedPubMedCentral

Zhang F, Jin Liu X, Qu X, Sheng Hu Z, Yang YM, Ma L et al (2012) Osteopontin’s colocalization with the adhesion molecule CEACAM5 in cytoplasm of carcinoma of tongue and its correlation with the invasion of that diease. Cancer Cell Int 12:33CrossRefPubMedPubMedCentral

Zhang M, Zhao K, Xu X, Yang Y, Yan S, Wei P et al (2018) A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma. 9:4475

Zhang X, Niu W, Mu M, Hu S, Niu C (2020) Long non-coding RNA LPP-AS2 promotes glioma tumorigenesis via miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop. Journal of experimental & clinical cancer research: CR. ;39:196

Zhao C, Guo R, Guan F (2020) MicroRNA-128-3p enhances the chemosensitivity of Temozolomide in Glioblastoma by Targeting c-Met and EMT. 10:9471

Zhou J, Fan X, Chen N, Zhou F, Dong J, Nie Y et al (2015) Identification of CEACAM5 as a biomarker for prewarning and prognosis in gastric Cancer. J Histochem cytochemistry: official J Histochem Soc 63:922–930CrossRef

Title: Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme
Authors: Renjie Wang
Qi Li
Xiaolei Chu
Nan Li
Haiqian Liang
Feng He
Publication date: 30-06-2023
Publisher: Springer US
Keywords: Glioblastoma
Glioblastoma
Glioblastoma
Published in: Metabolic Brain Disease / Issue 7/2023
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-023-01256-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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