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Journal of Assisted Reproduction and Genetics

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01-12-2017 | Reproductive Physiology and Disease

Evaluating lipopolysaccharide-induced oxidative stress in bovine granulosa cells

Authors: John J. Bromfield, Sossi M. Iacovides

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2017

Abstract

Purpose

The purpose of this study was to evaluate the capacity of bovine granulosa cells to generate reactive oxygen intermediates in response to lipopolysaccharide. We hypothesized that granulosa cells increase reactive oxygen intermediates in response to Gram-negative lipopolysaccharide in a similar manner to immune cells.

Methods

Bovine peripheral blood mononuclear cells and granulosa cells were cultured in the presence of lipopolysaccharide. Oxidative stress was evaluated using the fluorescent marker dye CellROX, and oxidative stress-related genes were measured using real-time RT-PCR.

Results

As expected, peripheral blood mononuclear cells increased oxidative stress in response to lipopolysaccharide as measured by accumulation of the fluorescent marker dye CellROX. While granulosa cells demonstrate the capacity to increase accumulation of CellROX dye in response to a positive control menadione, lipopolysaccharide had no effect on accumulation of CellROX dye. The expression of GSR, SOD1, and SOD2 were variable in peripheral blood mononuclear cells treated with lipopolysaccharide but were consistently upregulated when co-incubated with the antioxidant, N-acetyl cysteine. The expression of oxidative stressrelated genes was not altered in granulosa cells, with the exception of elevated SOD1 following lipopolysaccharide exposure in the absence of antioxidant.

Conclusions

Combined, these data suggest that while reactive stress is important in pathogen killing and inflammation in immune cells, granulosa cells do not increase oxidative stress in response to lipopolysaccharide.

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Title: Evaluating lipopolysaccharide-induced oxidative stress in bovine granulosa cells
Authors: John J. Bromfield
Sossi M. Iacovides
Publication date: 01-12-2017
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 12/2017
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-017-1031-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluating lipopolysaccharide-induced oxidative stress in bovine granulosa cells

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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