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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 9/2016

Open Access 01-09-2016 | Reproductive Physiology and Disease

Association of semen cytokines with reactive oxygen species and histone transition abnormalities

Authors: Lu Jiang, Ting Zheng, Jun Huang, Jinhua Mo, Hua Zhou, Min Liu, Xingcheng Gao, Bolan Yu

Published in: Journal of Assisted Reproduction and Genetics | Issue 9/2016

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Abstract

Purpose

The aim of this study is to investigate the relationships among reactive oxygen species (ROS) elevation, histone transition, and seminal cytokine concentrations.

Methods

Total levels of ROS in semen samples from 6560 men were measured. From this sample, 118 cases with high ROS and 106 controls were recruited. Basic semen parameters and histone-to-protamine ratios were analyzed, 400 semen cytokine and receptor alterations were assayed by protein chip, and finally 18 cytokines were validated in each sample using a Bio-Plex Cytokine assay.

Results

The results showed that the seminal ROS concentration was associated with abnormalities in the sperm histone transition. Compared with controls, 93 cytokines had significant alterations in the high ROS cases, with 14 of them further verified in individual samples. The concentrations of CXCL5, CXCL16, CXCL8, IL-1b, IL-10, CSF3, CCL3, and TNF-α were significantly correlated with the histone transition ratio. In addition, IL-16 showed significantly different concentrations in controls, normal semen with high ROS levels, and abnormal semen with high ROS levels.

Conclusions

Semen ROS are associated with abnormalities in sperm histone transition. CXCL5, CXCL8, IL-16, CCL8, CCL22, CCL20, CXCL16, IL-1B, IL-6, IL-7, IL-10, CSF3, CCL3, CCL4, and TNF-α all have elevated concentrations in semen with high ROS levels. These data might help to explain the mechanisms behind the increase in the levels of ROS and seminal cytokines and their relationship with defective spermatogenesis.
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Metadata
Title
Association of semen cytokines with reactive oxygen species and histone transition abnormalities
Authors
Lu Jiang
Ting Zheng
Jun Huang
Jinhua Mo
Hua Zhou
Min Liu
Xingcheng Gao
Bolan Yu
Publication date
01-09-2016
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 9/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-016-0756-7

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