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Journal of Assisted Reproduction and Genetics

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01-05-2015 | Embryo Biology

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Authors: Jin Huang, Nan Zhao, Xiaozhu Wang, Jie Qiao, Ping Liu

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2015

Abstract

Purpose

To investigate chromosomal characteristics in the same embryos at cleavage and blastocyst stage.

Methods

Six PGD/PGS cycles were retrospective studied, including five chromosomal translocation PGD cycles and one recurrent abortion PGS cycle. The cleavage embryos were biopsied one blastomere at day 3, followed by blastocyst culture. Trophectoderm cell biopsy was performed when embryos developed into the blastocyst stage. Whole genome amplification and 24-chromosomal analysis by CGH/SNP microarray was performed on each blastomere and trophectoderm cells.

Results

After PGD/PGS, only one couple had no euploid embryos to transfer. Five couples delivered a healthy, balanced-karyotype baby. A total of 18 embryos had both blastomere and trophectoderm cell reliable results available. Of the 18 embryos, eleven embryos contained identical chromosomes from cleavage stage to blastocyst stage and six embryos contained almost identical chromosomes . Only one embryo presented opposite chromosomal results for the cleavage and blastocyst stage, with abnormal chromosomes in the blastomere but normal chromosomes in trophectoderm cells.

Conclusions

Most embryos maintain chromosomal stability during embryonic development. Compared to cleavage stage, blastocyst stage may provide more reliable aneuploidy results.

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Title: Chromosomal characteristics at cleavage and blastocyst stages from the same embryos
Authors: Jin Huang
Nan Zhao
Xiaozhu Wang
Jie Qiao
Ping Liu
Publication date: 01-05-2015
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 5/2015
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-015-0450-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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