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Journal of Assisted Reproduction and Genetics

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01-06-2014 | Gamete Biology

The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model

Authors: S. Samuel Kim, Rachel Olsen, Dojun David Kim, David F. Albertini

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2014

Abstract

Purpose

To test the effects of varying vitrification protocols on the cell cycle status and chromosomal integrity in cumulus-enclosed GV stage rat oocytes.

Methods

Vitrified and thawed rat oocytes were labeled with fluorescent markers for chromatin, cell cycle activation, and f-actin and analyzed by conventional and laser scanning confocal microscopy.

Results

In all vitrification groups, significant alterations in cumulus cell connectivity, cell cycle status, and cytoplasmic actin integrity were observed following warming compared to fresh control oocytes. Based on the protein phosphorylation marker MPM-2, it is clear that warmed oocytes rapidly enter M-phase but are unable to maintain chromosome integrity as a result of multiple chromatin fusions. A prominent reduction in f-actin is evident in both the ooplasm and at the cortex of vitrified oocytes. Finally, an irreversible but irregular retraction of TZPs occurs on the majority of oocytes subjected to any of the vitrification protocols.

Conclusions

These findings draw attention to undesirable consequences of immature oocyte vitrification that compromise cell cycle status and chromatin and cytoskeleton integrity that may not be evident until after fertilization.

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Title: The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model
Authors: S. Samuel Kim
Rachel Olsen
Dojun David Kim
David F. Albertini
Publication date: 01-06-2014
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0216-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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