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Journal of Assisted Reproduction and Genetics

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01-08-2013 | ASSISTED REPRODUCTION TECHNOLOGIES

Effect of cumulus cell removal 4 h post-insemination on fertilization and embryo quality: a prospective randomized sibling-oocyte study

Authors: Yamei Xue, Xiaomei Tong, Lingying Jiang, Haiyan Zhu, Lingyun Yang, Songying Zhang

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2013

Abstract

Purpose

The study was designed to evaluate whether cumulus cell removal 4 h post-insemination could influence fertilization and embryo quality.

Methods

The study included 61couples undergoing standard long down regulation protocol from July 2011 to May 2012. Sibling oocytes of each patient were randomly assigned to either the 4 h group or the 20 group. For the 4 h group, cumulus cells were removed 4 h after gamete coincubation; for the 20 group, cumulus cells removal was performed 20 h after insemination. Fertilization rate, embryo quality, pregnancy rate and implantation rate were assessed.

Results

A total of 801 sibling cumulus-oocyte complexes (COCs) were randomized to the 4 h group (421 COCs) or 20 h group (380 COCs). There was no difference in the two pronuclei, one pronucleus and grade 1–2 embryo rate. Three pronuclei rate was significantly higher in the 4 h group compared to the 20 h group (12.6 % vs. 8.2 %, P = 0.041). Comparison of embryo transfer cycles in which either embryos from the 4 h group or 20 h group were transferred did not reveal any statistically significant differences in pregnancy or implantation rates.

Conclusion

The results of the present study indicate that cumulus cell removal 4 h post-insemination may increase the percentage of tripronuclear zygotes. However, normal fertilization rate, embryo development, clinical pregnancy rate and implantation rates are not influenced by the timing of cumulus cell removal.

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Title: Effect of cumulus cell removal 4 h post-insemination on fertilization and embryo quality: a prospective randomized sibling-oocyte study
Authors: Yamei Xue
Xiaomei Tong
Lingying Jiang
Haiyan Zhu
Lingyun Yang
Songying Zhang
Publication date: 01-08-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0049-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of cumulus cell removal 4 h post-insemination on fertilization and embryo quality: a prospective randomized sibling-oocyte study

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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