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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 12/2012

Open Access 01-12-2012 | Technological Innovations

Correlation between three assay systems for anti-Müllerian hormone (AMH) determination

Authors: Hang Wun Raymond Li, Ernest Hung Yu Ng, Benancy Po Chau Wong, Richard A. Anderson, Pak Chung Ho, William Shu Biu Yeung

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2012

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Abstract

Purpose

Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL).

Methods

A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L).

Results

We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R2 = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 × AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 × AMH (DSL) – 1.270 respectively.

Conclusions

AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended.
Literature
1.
Anderson RA, Nelson SM, Wallace WH. Measuring anti-Mullerian hormone for the assessment of ovarian reserve: when and for whom is it indicated? Maturitas. 2012;71(1):28–33.PubMedCrossRef
2.
Bersinger NA, Wunder D, Birkhäuser MH, Guibourdenche J. Measurement of anti-mullerian hormone by Beckman Coulter ELISA and DSL ELISA in assisted reproduction: differences between serum and follicular fluid. Clin Chim Acta. 2007;384:174–5.PubMedCrossRef
3.
Broer SL, Mol BWJ, Hendriks D, Broekmans FJM. The role of antimullerian hormone in prediction of outcome after IVF: comparison with the antral follicle count. Fertil Steril. 2009;91(3):705–14.PubMedCrossRef
4.
Broer SL, Dolleman M, Opmeer BC, Fauser BC, Mol BW, Broekmans FJ. AMH and AFC as predictors of excessive response in controlled ovarian hyperstimulation: a meta-analysis. Hum Reprod Update. 2011;17(1):46–54.PubMedCrossRef
5.
Freour T, Mirallie S, Bach-Ngohou K, Denis M, Barriere P, Masson D. Measurement of serum anti-mullerian hormone by Beckman Coulter ELISA and DSL ELISA: comparison and relevance in assisted reproduction technology (ART). Clin Chim Acta. 2007;375:162–4.PubMedCrossRef
6.
Hehenkamp WJK, Looman CWN, Themmen APN, de Jong FH, Te Velde ER, et al. Anti-Mullerian hormone levels in the spontaneous menstrual cycle do not show substantial fluctuation. J Clin Endocrinol Metab. 2006;91:4057–63.PubMedCrossRef
7.
Kini S, Li HWR, Morrell D, Pickering S, Thong KJ. Anti-mullerian hormone and cumulative pregnancy outcome in in-vitro fertilization. J Assist Reprod Genet. 2010;27(8):449–56.PubMedCrossRef
8.
Kumar A, Kalra B, Patel A, McDavid L, Roudebush WE. Development of a second generation anti-Mullerian hormone (AMH) ELISA. J Immunol Methods. 2010;362(1–2):51–9.PubMedCrossRef
9.
La Marca A, Stabile G, Artenisio AC, Volpe A. Serum anti-mullerian hormone throughout the human menstrual cycle. Hum Reprod. 2006;21:3103–7.PubMedCrossRef
10.
La Marca A, Broekmans FJ, Volpe A, Fauser BC, Macklon NS. Anti-Mullerian hormone (AMH): what do we still need to know? Hum Reprod. 2009;24(9):2264–75.PubMedCrossRef
11.
La Marca A, Nelson SM, Sighinolfi G, Manno M, Baraldi E, Roli L, Xella S, Marsella T, Tagliasacchi D, D'Amico R, et al. Anti-Mullerian hormone-based prediction model for a live birth in assisted reproduction. Reprod Biomed Online. 2011;22:341–9.PubMedCrossRef
12.
Lee MM, Donahoe PK, Hasegawa T, Silverman B, Crist GB, Best S, et al. Mullerian inhibiting substance in humans: normal levels from infancy to adulthood. J Clin Endocrinol Metab. 1996;81:571–6.PubMedCrossRef
13.
Lee JR, Kim SH, Jee BC, Suh CS, Kim KC, Moon SY. Antimullerian hormone as a predictor of controlled ovarian hyperstimulation outcome: comparison of two commercial immunoassay kits. Fertil Steril. 2011;95(8):2602–4.PubMedCrossRef
14.
Lekamge DN, Barry M, Kolo M, Lane M, Cilchrist RB, Tremellen KP. Anti-Mullerian hormone as a predictor of IVF outcome. Reprod BioMed Online. 2007;14:602–10.PubMedCrossRef
15.
Li HWR, Yeung WSB, Lau EYL, Ho PC, Ng EHY. Evaluating the performance of serum antimullerian hormone concentration in predicting the live birth rate of controlled ovarian stimulation and intrauterine insemination. Fertil Steril. 2010;94(6):2177–81.PubMedCrossRef
16.
Li HWR, Wong CYG, Yeung WSB, Ho PC, Ng EHY. Serum anti-Mullerian hormone level is not altered in women using hormonal contraceptives. Contraception. 2011;83(6):582–5.PubMedCrossRef
17.
Li HWR, Anderson RA, Yeung WSB, Ho PC, Ng EHY. Evaluation of serum anti-Mullerian hormone and inhibin B concentrations in the differential diagnosis of secondary oligo-amenorrhoea. Fertil Steril. 2011;96(3):774–9.PubMedCrossRef
18.
Nelson SM, Yates RW, Fleming R. Serum anti-Mullerian hormone and FSH: prediction of live birth and extremes of response in stimulated cycles–implications for individualization of therapy. Hum Reprod. 2007;22:2414–21.PubMedCrossRef
19.
Nelson SM, La Marca A. The journey from the old to the new AMH assay: how to avoid getting lost in the values. Reprod Biomed Online. 2011;23(4):411–20.PubMedCrossRef
20.
Streuli I, Fraisse T, Pillet C, Ibecheole V, Bischof P, de Ziegler D. Serum antimullerian hormone levels remain stable throughout the menstrual cycle and after oral or vaginal administration of synthetic sex steroids. Fertil Steril. 2008;90(2):395–400.PubMedCrossRef
21.
Streuli I, Fraisse T, Chapron C, Bijaoui G, Bischof P, de Ziegler D. Clinical uses of anti-Mullerian hormone assays: pitfalls and promises Fertil Steril. 2009;91:226–30.
22.
Wallace AM, Faye SA, Fleming R, Nelson SM. A multicentre evaluation of the new Beckman Coulter anti-Mullerian hormone (AMH) immunoassay (AMH Gen II). Ann Clin Biochem. 2011;48:370–3.PubMedCrossRef
Metadata
Title
Correlation between three assay systems for anti-Müllerian hormone (AMH) determination
Authors
Hang Wun Raymond Li
Ernest Hung Yu Ng
Benancy Po Chau Wong
Richard A. Anderson
Pak Chung Ho
William Shu Biu Yeung
Publication date
01-12-2012
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 12/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-012-9880-1

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