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Journal of Assisted Reproduction and Genetics

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01-05-2011 | Goanadal Physiology and Disease

The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population

Authors: Sonia Brahem, Meriem Mehdi, Hatem Elghezal, Ali Saad

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2011

Abstract

Purpose

To investigate the effects of male aging on semen quality, DNA fragmentation and chromosomal abnormalities in the spermatozoa of infertile patients and fertile men.

Methods

Semen samples of 140 infertile patients (24–76 years) and 50 men with proven fertility (25–65 years) were analyzed according to WHO guidelines. DNA fragmentation was detected by TUNEL assay, while aneuploidy was assessed by FISH.

Results

In the patient group, semen volume and vitality of spermatozoa decreased significantly with age, while sperm concentration showed a statistically significant increase with age. DNA fragmentation as well as disomy of sex chromosomes and disomy 8 did not show a statistically significant change with age. However, the diploidy rate was significantly increased with patient’s age. In the control group, conventional semen parameters as well as DNA fragmentation and chromosomal abnormalities did not show a statistically significant with age.

Conclusion

Increased age in infertile men is associated with an increase in sperm concentration and diploidy, as well as a decline in semen volume and sperm vitality. However motility, morphology and DNA fragmentation are not affected by male age.

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Title: The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population
Authors: Sonia Brahem
Meriem Mehdi
Hatem Elghezal
Ali Saad
Publication date: 01-05-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 5/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9537-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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