Top

Journal of Assisted Reproduction and Genetics

Published in:

01-03-2011 | Assisted Reproduction Technologies

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Authors: Martin Wilding, Gianfranco Coppola, Loredana di Matteo, Antonio Palagiano, Enrico Fusco, Brian Dale

Published in: Journal of Assisted Reproduction and Genetics | Issue 3/2011

Abstract

Purpose

We used computer assisted sperm selection (MSOME) during cycles of intracytoplasmic sperm injection to test whether this technique improves results over traditional ICSI protocols. We also used the TUNEL assay to test whether MSOME could deselect physiologically abnormal spermatozoa.

Methods

Individual spermatozoa were examined with MSOME. Normal and abnormal spermatozoa were tested for the level of DNA fragmentation using TUNEL assay. In a prospective, randomized trial, patients were selected for standard ICSI, or IMSI techniques. We tested the two groups for biological and clinical parameters.

Results

64.8% of spermatozoa, otherwise selectable for ICSI, were characterized by abnormalities after computer-assisted sperm analysis. These sperm were also characterized by an increase in the level of DNA fragmentation. We noted an increase in embryo quality, pregnancy and implantation rates after computerized sperm selection during ICSI procedures.

Conclusions

Computerised selection of spermatozoa during ICSI procedures deselects physiological abnormal spermatozoa and improves clinical results.

Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–8.PubMedCrossRef

Palermo GD, Cohen J, Alikani M, Adler A, Rosenwaks Z. Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil Steril. 1995;63:1231–40.PubMed

Lim AS, Tsakok MF. Age-related decline in fertility: a link to degenerative oocytes? Fertil Steril. 1997;68:265–71.PubMedCrossRef

Sherins RJ, Thorsell LP, Dorfmann A, Dennison-Lagos L, Calvo LP, Krysa L, et al. Intracytoplasmic sperm injection facilitates fertilization even in the most severe forms of male infertility: pregnancy outcome correlates with maternal age and number of eggs available. Fertil Steril. 1995;64:369–75.PubMed

Sauer MV. The impact of age on reproductive potential: lessons learned from oocyte donation. Maturitas. 1998;30:221–5.PubMedCrossRef

Kini S, Morrell D, Thong KJ, Kopakaki A, Hillier S, Irvine DS. Lack of impact of semen quality on fertilization in assisted conception. Scott Med J. 2010;55:20–3.PubMedCrossRef

Campbell AJ, Irvine DS. Male infertility and intracytoplasmic sperm injection (ICSI). Br Med Bull. 2000;56:616–29.PubMedCrossRef

Ménézo Y, Dale B. Paternal contribution to successful embryogenesis. Hum Reprod. 1995;10:1326–8.PubMed

Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl. 2002;23:1–8.PubMed

10.

Berkovitz A, Eltes F, Ellenbogen A, Peer S, Feldberg D, Bartoov B. Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome? Hum Reprod. 2006;21:1787–90.PubMedCrossRef

11.

Berkovitz A, Eltes F, Lederman H, Peer S, Ellenbogen A, Feldberg B, et al. How to improve IVF-ICSI outcome by sperm selection. Reprod Biomed Online. 2006;12:634–8.PubMedCrossRef

12.

Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R, et al. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril. 2009;92:1616–25.PubMedCrossRef

13.

Chelli MH, Albert M, Ray PF, Guthauser B, Izard V, Hammoud I, et al. Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome? Fertil Steril. 2010;93:1347. e1-5.PubMedCrossRef

14.

Oliveira JB, Massaro FC, Mauri AL, Petersen CG, Nicoletti AP, Baruffi RL, et al. Motile sperm organelle morphology examination is stricter than Tygerberg criteria. Reprod Biomed Online. 2009;18:320–6.PubMedCrossRef

15.

Berkovitz A, Eltes F, Yaari S, Katz N, Barr I, Fishman A, et al. The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod. 2005;20:185–90.PubMedCrossRef

16.

Bartoov B, Berkovitz A, Eltes F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001;345:1067–8.PubMedCrossRef

17.

Nadalini M, Tarozzi N, Distratis V, Scaravelli G, Borini A. Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reprod Biomed Online. 2009;19 Suppl 3:45–55.PubMedCrossRef

18.

Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, et al. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80:1413–9.PubMedCrossRef

19.

Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P, Tesarik J. High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online. 2006;12:19–25.PubMedCrossRef

20.

Antinori M, Licata E, Dani G, Cerusico F, Versaci C, d’Angelo D, et al. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online. 2008;16:835–41.PubMedCrossRef

21.

Kacem O, Sifer C, Barraud-Lange V, Ducot B, De Ziegler D, Poirot C, et al. Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin. Reprod Biomed Online. 2010;20:132–7.PubMedCrossRef

22.

Collins JA, Barnhart KT, Schlegel PN. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? Fertil Steril. 2008;89:823–31.PubMedCrossRef

23.

Benchaib M, Lornage J, Mazoyer C, Lejeune H, Salle B, François Guerin J. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril. 2007;87:93–100.PubMedCrossRef

24.

Meseguer M, Santiso R, Garrido N, García-Herrero S, Remohí J, Fernandez JL. Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. Fertil Steril. 2010;(in press).

25.

De Placido G, Wilding M, Strina I, Alviggi E, Alviggi C, Mollo A, et al. High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate. Hum Reprod. 2002;17:2402–9.PubMedCrossRef

26.

Wen SW, Leader A, White RR, Léveillé MC, Wilkie V, Zhou J, et al. A comprehensive assessment of outcomes in pregnancies conceived by in vitro fertilization/intracytoplasmic sperm injection. Eur J Obstet Gynecol Reprod Biol. 2010;150:160–5.PubMedCrossRef

27.

Hindryckx A, Peeraer K, Debrock S, Legius E, de Zegher F, Francois I, et al. Has the Prevalence of Congenital Abnormalities after Intracytoplasmic Sperm Injection Increased? The Leuven Data 1994 –2000 and a Review of the Literature. Gynecol Obstet Invest. 2010;70:11–22.PubMedCrossRef

28.

Ludwig AK, Hansen A, Katalinic A, Sutcliffe AG, Diedrich K, Ludwig M. Thyen Ute. Assessment of vision and hearing in children conceived spontaneously and by ICSI: a prospective controlled, single-blinded follow-up study. Reprod Biomed Online. 2010;20:391–7.PubMedCrossRef

29.

Wilding M, Di Matteo L, D'Andretti S, Montanaro N, Capobianco C, Dale B. An oocyte score for use in assisted reproduction. J Assist Reprod Genet. 2007;24:350–8.PubMedCrossRef

30.

Ubaldi F, Rienzi L. Morphological selection of gametes. Placenta. 2008;29(Suppl B):115–20.PubMedCrossRef

31.

Sartorius GA, Nieschlag E. Paternal age and reproduction. Hum Reprod Update. 2010;16:65–79.PubMedCrossRef

32.

Sathananthan AH. Functional competence of abnormal spermatozoa. Baillieres Clin Obstet Gynaecol. 1994;8:141–56.PubMedCrossRef

33.

Frattarelli JL, Leondires MP, Miller BT, Segars JH. Intracytoplasmic sperm injection increases embryo fragmentation without affecting clinical outcome. J Assist Reprod Genet. 2000;17:207–12.PubMedCrossRef

Title: Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa
Authors: Martin Wilding
Gianfranco Coppola
Loredana di Matteo
Antonio Palagiano
Enrico Fusco
Brian Dale
Publication date: 01-03-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 3/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9505-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 3/2011

Assisted reproduction in a patient with Klippel-Trenaunay syndrome: management of thrombophilia and consumptive coagulopathy

Influence of gene variants related to calcium homeostasis on biochemical parameters of women with polycystic ovary syndrome

Association of progesterone receptor polymorphism with idiopathic recurrent pregnancy loss in Taiwanese Han population

Preimplantation genetic diagnosis (PGD) for SHOX-related haploinsufficiency in conjunction with trisomy 21 detection by molecular analysis

A shared egg donor program: which is the minimum number of oocytes to be allocated?

There is no complete linkage between the polymorphisms N680S and T307A of the follicular stimulating hormone receptor gene in fertile women