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Journal of Assisted Reproduction and Genetics

Published in:

01-02-2011 | Assisted Reproduction Technologies

Correlation between embryological factors and pregnancy rate: development of an embryo score in a cryopreservation programme

Authors: Miquel Solé, Josep Santaló, Ignacio Rodríguez, Montse Boada, Buenaventura Coroleu, Pere N. Barri, Anna Veiga

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2011

Abstract

Purpose

To establish which embryo parameters, in frozen thawed embryo transfers, have the highest prognosis value in the establishment of pregnancy. The relative importance of different embryo parameters is used to develop an embryo score.

Methods

Retrospective analysis of the implantation rate in 356 frozen/thawed single embryo transfers. A logistic regression model is used to establish an embryo score.

Results

A direct correlation is established between the implantation rate and fresh embryo development (number of blastomeres and their symmetry), survival rate after thawing and mitosis resumption after overnight culture.

Conclusions

An embryo score is developed to determine the implantation potential of frozen/thawed embryos.

Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature. 1983;305:707–9.PubMedCrossRef

Zeilmaker GH, Alberda AT, van Gent I, Rijkmans CM, Drogendijk AC. Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril. 1984;42:293–6.PubMed

Veiga A, Calderon G, Barri PN, Coroleu B. Pregnancy after the replacement of a frozen-thawed embryo with less than 50% intact blastomeres. Hum Reprod. 1987;2:321–3.PubMed

Tiitinen A, Halttunen M, Harkki P, Vuoristo P, Hyden-Granskog C. Elective single embryo transfer: the value of cryopreservation. Hum Reprod. 2001;16:1140–4.PubMedCrossRef

Le Lannou D, Griveau JF, Laurent MC, Gueho A, Veron E, Morcel K. Contribution of embryo cryopreservation to elective single embryo transfer in IVF-ICSI. Reprod Biomed Online. 2006;13:368–75.PubMedCrossRef

Lundin K, Bergh C. Cumulative impact of adding frozen-thawed cycles to single versus double fresh embryo transfers. Reprod Biomed Online. 2007;15:76–82.PubMedCrossRef

Sills ES, McLoughlin LJ, Genton MG, Walsh DJ, Coull GD, Walsh AP. Ovarian hyperstimulation syndrome and prophylactic human embryo cryopreservation: analysis of reproductive outcome following thawed embryo transfer. J Ovarian Res. 2008;1:7.PubMedCrossRef

Verwoerd GR, Mathews T, Brinsden PR. Optimal follicle and oocyte numbers for cryopreservation of all embryos in IVF cycles at risk of OHSS. Reprod Biomed Online. 2008;17:312–7.PubMedCrossRef

Edgar DH, Bourne H, Jericho H, McBain JC. The developmental potential of cryopreserved human embryos. Mol Cell Endocrinol. 2000;169:69–72.PubMedCrossRef

10.

Andersen AN, Gianaroli L, Felberbaum R, de Mouzon J, Nygren KG, The European IVF-monitoring programme (EIM), European Society of Human Reproduction and Embryology (ESHRE). Assisted reproductive technology in Europe, 2001. Results generated from European registers by ESHRE. Hum Reprod 2005;20:1158–76.

11.

Schalkoff ME, Oskowitz SP, Powers RD. A multifactorial analysis of the pregnancy outcome in a successful embryo cryopreservation program. Fertil Steril. 1993;59:1070–4.PubMed

12.

Edgar DH, Jericho H, Bourne H, McBain JC. The influence of prefreeze growth rate and blastomere number on cryosurvival and subsequent implantation of human embryos. J Assist Reprod Genet. 2001;18:135–8.PubMedCrossRef

13.

Salumets A, Tuuri T, Makinen S, Vilska S, Husu L, Tainio R, et al. Effect of developmental stage of embryo at freezing on pregnancy outcome of frozen-thawed embryo transfer. Hum Reprod. 2003;18:1890–5.PubMedCrossRef

14.

El-Toukhy T, Khalaf Y, Al-Darazi K, Andritsos V, Taylor A, Braude P. Effect of blastomere loss on the outcome of frozen embryo replacement cycles. Fertil Steril. 2003;79:1106–11.PubMedCrossRef

15.

Gabrielsen A, Fedder J, Agerholm I. Parameters predicting the implantation rate of thawed IVF/ICSI embryos: a retrospective study. Reprod Biomed Online. 2006;12:70–6.PubMedCrossRef

16.

Tang R, Catt J, Howlett D. Towards defining parameters for a successful single embryo transfer in frozen cycles. Hum Reprod. 2006;21:1179–83.PubMedCrossRef

17.

Salumets A, Suikkari AM, Makinen S, Karro H, Roos A, Tuuri T. Frozen embryo transfers: implications of clinical and embryological factors on the pregnancy outcome. Hum Reprod. 2006;21:2368–74.PubMedCrossRef

18.

Edgar DH, Archer J, McBain J, Bourne H. Embryonic factors affecting outcome from single cryopreserved embryo transfer. Reprod Biomed Online. 2007;14:718–23.PubMedCrossRef

19.

Lassalle B, Testart J, Renard JP. Human embryo features that influence the success of cryopreservation with the use of 1, 2 propanediol. Fertil Steril. 1985;44:645–51.PubMed

20.

Coroleu B, Barri PN, Carreras O, Martinez F, Veiga A, Balasch J. The usefulness of ultrasound guidance in frozen-thawed embryo transfer: a prospective randomized clinical trial. Hum Reprod. 2002;17:2885–90.PubMedCrossRef

21.

Hosmer DW, Lemeshow S. Applied logistic regression. 2nd ed. New York: Willey; 2000.CrossRef

22.

Hyden-Granskog C, Unkila-Kallio L, Halttunen M, Tiitinen A. Single embryo transfer is an option in frozen embryo transfer. Hum Reprod. 2005;20:2935–8.PubMedCrossRef

23.

Olivius C, Lundin K, Bergh C. Predictive factors for live birth in cryopreservation single embryo transfer cycles. Reprod Biomed Online. 2008;17:676–83.PubMedCrossRef

24.

Mandelbaum J, Belaisch-Allart J, Junca AM, Antoine JM, Plachot M, Alvarez S, et al. Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes. Hum Reprod. 1998;Suppl 3:161–74. discussion 175–7.

25.

Lahav-Baratz S, Koifman M, Shiloh H, Ishai D, Wiener-Megnazi Z, Dirnfeld M. Analyzing factors affecting the success rate of frozen-thawed embryos. J Assist Reprod Genet. 2003;20:444–8.PubMedCrossRef

26.

Sifer C, Sellami A, Poncelet C, Martin-Pont B, Porcher R, Hugues JN, et al. Day 3 compared with day 2 cryopreservation does not affect embryo survival but improves the outcome of frozen-thawed embryo transfers. Fertil Steril. 2006;86:1537–40.PubMedCrossRef

27.

Hardarson T, Hanson C, Sjogren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod. 2001;16:313–8.PubMedCrossRef

28.

Holte J, Berglund L, Milton K, Garello C, Gennarelli G, Revelli A, et al. Construction of an evidence-based integrated morphology cleavage embryo score for implantation potential of embryos scored and transferred on day 2 after oocyte retrieval. Hum Reprod. 2007;22:548–57.PubMedCrossRef

29.

Van Royen E, Mangelschots K, De Neubourg D, Valkenburg M, Van de Meerssche M, Ryckaert G, et al. Characterization of a top quality embryo, a step towards single-embryo transfer. Hum Reprod. 1999;14:2345–9.PubMedCrossRef

30.

Guerif F, Bidault R, Cadoret V, Couet ML, Lansac J, Royere D. Parameters guiding selection of best embryos for transfer after cryopreservation: a reappraisal. Hum Reprod. 2002;17:1321–6.PubMedCrossRef

Title: Correlation between embryological factors and pregnancy rate: development of an embryo score in a cryopreservation programme
Authors: Miquel Solé
Josep Santaló
Ignacio Rodríguez
Montse Boada
Buenaventura Coroleu
Pere N. Barri
Anna Veiga
Publication date: 01-02-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9498-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Correlation between embryological factors and pregnancy rate: development of an embryo score in a cryopreservation programme

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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