Published in:
Open Access
01-06-2010 | Embryo biology
Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints
Authors:
Ann A. Kiessling, Ritsa Bletsa, Bryan Desmarais, Christina Mara, Kostas Kallianidis, Dimitris Loutradis
Published in:
Journal of Assisted Reproduction and Genetics
|
Issue 6/2010
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Abstract
Purpose
To understand cell cycle controls in the 8-Cell human blastomere.
Methods
Data from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripotency, and embryonic stem cells. A sub database of 3,803 genes identified by high throughput RNA knock-down studies, plus genes that oscillate in human cells, was analyzed.
Results
Thirty-five genes over-detected at least 7-fold specifically on the 8-Cell arrays were enriched for cell cycle drivers and for proteins that stabilize chromosome cohesion and spindle attachment and limit DNA and centrosome replication to once per cycle.
Conclusions
These results indicate that 8-cell human blastomere cleavage is guided by cyclic over-expression of key proteins, rather than canonical checkpoints, leading to rapidly increasing gene copy number and a susceptibility to chromosome and cytokinesis mishaps, well-noted characteristics of preimplantation human embryos.