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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 6/2010

01-06-2010 | Embryo Biology

Kinetics of human male pronuclear development in a heterologous ICSI model

Authors: Estella L. Jones, Olga Mudrak, Andrei O. Zalensky

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2010

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Abstract

Purpose

To evaluate human sperm nuclear chromatin decondensation in a heterologous ICSI system using hamster ova injected with human sperm.

Materials and methods

Frozen hamster oocytes were injected with Triton X-100 treated sperm and fixed at different time points post ICSI. Oocytes injected with non-treated sperm served as controls. Male pronuclear decondensation was evaluated after staining with DAPI.

Results

Sperm cells with partially destroyed membranes and depletion of the acrosome decondense more rapidly and to a greater extent than membrane/acrosome intact cells. Marked variability in pronuclear size was observed for any time point post ICSI, which most probably reflects the heterogeneity in the mature human sperm population.

Conclusion

Remodeling of male gamete nuclei in this heterologous ICSI mimics events that occur during natural fertilization in humans and therefore this approach may be used for studies of human sperm chromosomes transformations.
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Metadata
Title
Kinetics of human male pronuclear development in a heterologous ICSI model
Authors
Estella L. Jones
Olga Mudrak
Andrei O. Zalensky
Publication date
01-06-2010
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 6/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9402-y

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