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Journal of Assisted Reproduction and Genetics

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01-02-2010 | ANDROLOGY

Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa

Authors: Amjad Hossain, Collin Osuamkpe, Shaikat Hossain, John Y. Phelps

Published in: Journal of Assisted Reproduction and Genetics | Issue 2-3/2010

Abstract

Purpose

To investigate the prevalence of spontaneously developed tail swellings (SDTS) in human sperm samples that are commonly encountered in the laboratory, and their influence on the hypo-osmotic swelling test (HOS-test).

Methods

Ejaculated, epididymal, and testicular sperm were evaluated for SDTS. Further, HOS-test scores were compared with those of vital stains using column washed sperm maintained in the laboratory.

Results

SDTS, at <10%, was present in all types of sperm samples. The highest and lowest occurrences of SDTS were found in cryopreserved sperm, and column-washed sperm respectively. SDTS can inflate the HOS-test score, and so lower the accuracy of the HOS-test. However, the HOS-test efficiency can be improved by assessing SDTS in the sample.

Conclusion

HOS-test and vital stain cannot be used interchangeably in all circumstances for sperm viability determination. The accuracy of the HOS-test can be enhanced by incorporating SDTS as a correction factor.

Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil. 1984;70:219–28.PubMedCrossRef

Schrader SM, Platek SF, Zaneveld LJ, Perez-Pelaez M, Jeyendran RS. Sperm viability: a comparison of analytical methods. Andrologia. 1986;18:530–8.PubMed

Munuce MJ, Caille AM, Perfumo P, Morisoli L. Does the hypo-osmotic swelling test predict human sperm viability? Arch Androl. 2000;44:207–12.CrossRefPubMed

Desmet B, Joris H, Nagy Z, Liu J, Bocken G, Vankelecom A, et al. Selection of vital immotile spermatozoa for intracytoplasmic injection by the hypo-osmotic swelling test. 10^th Annual Meeting of ESHRE, June 1994. Hum Reprod. 1994;9(Suppl. 4): p. 24.

Casper RF, Cowan L, Lucato ML, Jarvi KA. The hypoosmotic swelling test for the selection of viable sperm for inctracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65:972–6.PubMed

Liu J, Tsai YL, Katz E, Compton G, Garcia JE, Baramki TA. High fertilization rate obtained after intracytoplasmic sperm injection with 100% non-motile spermatozoa selected by using a simple modified hypo-osmotic swelling test. Fertil Steril. 1997;68:373–5.CrossRefPubMed

Smikle CB, Turek PJ. Hypo-osmotic swelling can accurately assess the viability of nonmotile sperm. Mol Reprod Develop. 1997;47:200–3.CrossRef

Lin MH, Morshedi M, Srisombut C, Nassar A, Oehninger S. Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water tests, and eosin-Y staining. Fertil Steril. 1998;70:1148–55.CrossRefPubMed

Jager S, Kremer J, Mijchman J. Hypoosmotic sperm swelling test does not assess fertilizing capacity of human spermatozoa. Arch Androl. 1991;26:195–7.CrossRefPubMed

10.

Esteves SC, Sharma RK, Thomas AJ, Agarwal A. Suitability of the hypo-osmotic swelling test for assessing the viability of cryopreserved sperm. Fertil Steril. 1996;66:798–804.PubMed

11.

Avery S, Bolton VN, Mason BA. An evaluation of the hypo-osmotic sperm swelling test as a predictor of fertilizing capacity in vitro. Int J Androl. 1990;13:93–9.CrossRefPubMed

12.

Martini AC, Estofan D, Ruiz R, Cuneo M. Improving the predictive value of the hypo-osmotic swelling test in humans. Fertil Steril. 2006;85:1840–2.CrossRefPubMed

13.

World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University; 1999.

14.

Gardner DK, Weissman A, Howles CM, Shoham Z. Textbook of assisted reproductive techniques: laboratory and clinical perspectives. 1st ed. London: Dunitz; 2001.

15.

Rossato M, Balercia G, Lucarelli G, Mantero F. Role of seminal osmolarity in the regulation of human sperm motility. Int J Androl. 2002;37:207–18.

16.

Hossain A, Osuamkpe C, Nagamani M. Extended culture of human spermatozoa in the laboratory may have practical value in the assisted reproductive procedures. Fertil Steril. 2007;89:237–9.CrossRefPubMed

17.

Misro M, Chaki S. Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm. Fertil Steril. 2008;89:223–7.CrossRefPubMed

Title: Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa
Authors: Amjad Hossain
Collin Osuamkpe
Shaikat Hossain
John Y. Phelps
Publication date: 01-02-2010
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2-3/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9375-x

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Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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