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Journal of Assisted Reproduction and Genetics

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Open Access 01-04-2009 | GENETICS

Evidence that human blastomere cleavage is under unique cell cycle control

Authors: Ann A. Kiessling, Ritsa Bletsa, Bryan Desmarais, Christina Mara, Kostas Kallianidis, Dimitris Loutradis

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2009

Abstract

Purpose

To understand the molecular pathways that control early human embryo development.

Methods

Improved methods of linear amplification of mRNAs and whole human genome microarray analyses were utilized to characterize gene expression in normal appearing 8-Cell human embryos, in comparison with published microarrays of human fibroblasts and pluripotent stem cells.

Results

Many genes involved in circadian rhythm and cell division were over-expressed in the 8-Cells. The cell cycle checkpoints, RB and WEE1, were silent on the 8-Cell arrays, whereas the recently described tumor suppressor, UHRF2, was up-regulated >10-fold, and the proto-oncogene, MYC, and the core element of circadian rhythm, CLOCK, were elevated up to >50-fold on the 8-Cell arrays.

Conclusions

The canonical G1 and G2 cell cycle checkpoints are not active in totipotent human blastomeres, perhaps replaced by UHRF2, MYC, and intracellular circadian pathways, which may play important roles in early human development.

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Title: Evidence that human blastomere cleavage is under unique cell cycle control
Authors: Ann A. Kiessling
Ritsa Bletsa
Bryan Desmarais
Christina Mara
Kostas Kallianidis
Dimitris Loutradis
Publication date: 01-04-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 4/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9306-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evidence that human blastomere cleavage is under unique cell cycle control

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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