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Journal of Assisted Reproduction and Genetics

Published in:

01-08-2007 | Assisted Reproduction

An oocyte score for use in assisted reproduction

Authors: Martin Wilding, Loredana Di Matteo, Sonia D’Andretti, Nadia Montanaro, Clemente Capobianco, Brian Dale

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2007

Abstract

Purpose

In this work, we describe a system for the morphological scoring of human oocytes prior to fertilisation and use this system to test whether oocyte morphology is an indicator of fertilisation, embryo development and implantation potential.

Methods

The study is a prospective trial of the use of oocyte morphological scores in 822 patients undergoing their first cycle of ICSI. Analyses of oocytes were performed prior to ICSI procedures and the scores compared with fertilisation rates, embryo quality and clinical results.

Results

‘Top quality’ oocytes had a significantly higher level of fertilisation (96%) as compared to low scoring oocytes (25.6%). Where top quality oocytes formed top quality embryos, we noted a clinical success rate of 63.4%.

Conclusions

Clinical success rates were increased in cases where top quality oocytes formed top quality embryos after ICSI. The analysis of oocyte morphology may represent a positive selection feature during ICSI.

Coetsier T, Dhont M. Avoiding multiple pregnancies in in-vitro fertilisation: who’s afraid of single embryo transfer? Hum Reprod 1998;10:2663–70.

Pennings G. Multiple pregnancies: a test case for the moral quality of assisted reproduction. Hum Reprod 2000;15:2466–9.PubMedCrossRef

Gleicher N, Barad D. The relative myth of elective single embryo transfer. Hum Reprod 2006;21:1337–44.PubMedCrossRef

Wang J, Lane M, Norman RJ. Reducing multiple pregnancy from assisted reproduction treatment: educating patients and medical staff. Med J Aust 2006;184:180–1.PubMed

Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft W. Culture of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil Steril 1998;69:84–8.PubMedCrossRef

Gardner DK, Schoolcraft W. Elimination of higher order multiple gestations by blastocyst culture and transfer. In: Shoham Z, Howles C, Jacobs H, editors. Elimination of higher order multiple gestations by blastocyst culture and transfer. London: Martin Dunitz; 1999. p. 267–74.

Edwards RG, Fishel SB, Cohen J, Fehilly CB, Purdy JM, Slater JM, et al. Factors influencing the success of in vitro fertilisation for alleviating human infertility. J In Vitro Fertil Embryo Transf 1984;1:3–23.CrossRef

Cummins JM, Breen TM, Harrison KL, Shaw JM, Wilson LM, Hennessey JF. A formula for scoring human embryo growth rates in in vitro fertilisation: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J In Vitro Fertil Embryo Transf 1986;3:284–95.CrossRef

Puissant F, Van Rysselberge M, Barlow P, Dewesze J, Leroy F. Embryo scoring as a prognostic tool in IVF treatment. Hum Reprod 1987;2:705–8.PubMed

10.

Visser D, Fourie F. The applicability of cumulative embryo score selection and quality control in an in-vitro fertilisation/embryo transfer programme. Hum Reprod 1993;8:1719–22.PubMed

11.

Giorgetti C, Terriou P, Auquier P, Hans E, Spach JL, Salzmann J, et al. Embryo score to predict implantation after in vitro fertilisation: based on 957 single embryo transfers. Hum Reprod 1995;10:2427–31.PubMed

12.

Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilisation. Hum Reprod 1997;12:1545–9.PubMedCrossRef

13.

Rjinders P, Jansen C. The predictive value of day 3 embryo morphology regarding blastocysts formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilisation or intracytoplasmic sperm injection. Hum Reprod 1998;13:2869–73.

14.

Hu Y, Maxson WS, Hoffman DI, Ory SJ, Eager S, Dupre J, et al. Maximising pregnancy rates and limiting high-order multiple conceptions by determining the optimal number of embryos to transfer based on quality. Fertil Steril 1998;69:650–7.PubMedCrossRef

15.

Alikani M, Calderon G, Tomkin G, Garrisi J, Kokot M, Cohen J. Cleavage anomalies in early human embryos and survival after prolonged culture in vitro. Hum Reprod 2000;15:2634–43.PubMedCrossRef

16.

Alikani M, Cohen J, Tomkin G, Garrisi J, Mack C, Scott RT. Human embryo fragmentation in-vitro and its effects for human pregnancy and implantation. Fertil Steril 1999;71:836–42.PubMedCrossRef

17.

Desai N, Goldstein J, Rowland D, Goldfarb J. Morphological evaluation of human embryos and derivation of an embryo quality scoring system specific for day 3 embryos: a preliminary study. Hum Reprod 2000;15:2190–6.PubMedCrossRef

18.

Racowsky C, Jackson KV, Cekleniak NA, Fox JH, Hornstein MD, Ginsberg EJ. The number of eight-cell embryos is a key determinant for selecting day 3 or day 5 transfer. Fertil Steril 2000;73:558–64.PubMedCrossRef

19.

Hardarson T, Hanson C, Sjogren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod 2001;16:313–8.PubMedCrossRef

20.

Langley MT, Marek DM, Gardner DK, Doody KM, Doody KJ. Extended embryo culture in human assisted reproduction treatments. Hum Reprod 2001;16:902–8.PubMedCrossRef

21.

Hamamah S. Oocyte and embryo quality: is their morphology a good criterion? J Gynecol Obstet Biol Reprod (Paris) 2005;34:5S38–41.

22.

Edwards RG, Steptoe PC, Purdy JM. Establishing full-term human pregnancies using cleaving embryos grown in vitro. Br J Obstet Gynaecol 1980;87:737–56.PubMed

23.

Sadowy S, Tomkin G, Munne S, Ferrara-Congedo T, Cohen J. Impaired development of zygotes with uneven pronuclear size. Zygote 1998;6:137–42.PubMedCrossRef

24.

Tesarik J, Greco E. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Hum Reprod 1999;14:1318–23.PubMedCrossRef

25.

Scott L, Alvero R, Leondires M, Miller B. The morphology of human pronuclear embryos is positively related to blastocysts development and implantation. Hum Reprod 2000;15:2394–403.PubMedCrossRef

26.

Tesarik J, Junca AM, Hazout A, Aubriot FX, Nathan C, Cohen-Bacrie P, et al. Embryos with high implantation potential after intracytoplasmic sperm injection can be recognised by a simple, non-invasive examination of pronuclear morphology. Hum Reprod 2000;15:1396–9.PubMedCrossRef

27.

Wittemer C, Bettahar-Lebugle K, Ohl J, Rongieres C, Nisand I, Gerlinger P. Zygote evaluation: an efficient tool for embryo selection. Hum Reprod 2000;15:2591–7.PubMedCrossRef

28.

Ludwig M, Schopper B, Al-Hasani S, Diedrich K. Clinical use of a pronuclear stage score following intracytoplasmic sperm injection: impact on pregnancy rates under the conditions of the German embryo law. Hum Reprod 2000;15:325–9.PubMedCrossRef

29.

Dale B, Fiorentino A, de Simone ML, di Matteo L, Scotto di Frega A, Wilding M, et al. Zygote versus embryo transfer: a prospective randomized multicenter trial. J Assist Reprod Genet 2002;19:456–61.PubMedCrossRef

30.

Payne JF, Raburn DJ, Couchman GM, Price TM, Jamison MG, Walmer DK. Relationship between pre-embryo pronuclear morphology (zygote score) and standard day 2 or 3 embryo morphology with regard to assisted reproductive technique outcomes. Fertil Steril 2005;84:900–9.PubMedCrossRef

31.

Chen C, Kattera S. Comparison of pronuclear zygote morphology and early cleavage status of zygotes as additional criteria in the selection of day 3 embryos: a randomized study. Fertil Steril 2006;85:347–52.PubMedCrossRef

32.

Senn A, Urner F, Chanson A, Primi MP, Wirthner D, Germond M. Morphological scoring of human pronuclear zygotes for prediction of pregnancy outcome. Hum Reprod 2006;21:234–9.PubMedCrossRef

33.

Gesetz zum Schutz von Embryonen. In ‘Fassung der Bekanntmachung’ 13. Dezember 1990–BGBl. I S. 2747.

34.

LPAM Legge federale del 18 Dicembre 1998 concernente la procreazione con assistenza medica (Legge sulla medicina della procreazione). RU 2000 3055.

35.

Legge 40 “Norme in materia di procreazione medicalmente assistita”. Gazzetta Ufficiale Repubblica Italiana 2004, 45 del 24 Febbraio.

36.

De Sutter P, Dozortsev D, Qian C, Dhont M. Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod 1996;11:595–7.PubMed

37.

Sathananthan AH. Ultrastructure of the human egg. Hum Cell 1997;10:21–38.PubMed

38.

Serhal PF, Ranieri DM, Kinis A, Marchant S, Davies M, Khadum IM. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod 1997;12:1267–70.PubMedCrossRef

39.

Loutradis D, Drakakis P, Kallianidis K, Milingos S, Dendrinos S, Michalas S. Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection. Fertil Steril 1999;72:240–4.PubMedCrossRef

40.

Suppinyopong S, Choavaratana R, Karavakul C. Correlation of oocyte morphology with fertilization rate and embryo quality after intracytoplasmic sperm injection. J Med Assoc Thail 2000;83:627–32.

41.

Plachot M, Selva J, Wolf JP, Bastit P, de Mouzon J. Consequences of oocyte dysmorphy on the fertilization rate and embryo development after intracytoplasmic sperm injection. A prospective multicenter study. Gynecol Obstet Fertil 2002;30:772–9.PubMedCrossRef

42.

Otsuki J, Okada A, Morimoto K, Nagai Y, Kubo H. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod 2004;19:1591–7.PubMedCrossRef

43.

Ebner T, Moser M, Tews G. Is oocyte morphology prognostic of embryo developmental potential after ICSI? Reprod Biomed Online 2006;12:507–12.PubMedCrossRef

44.

Van Blerkom J, Henry G. Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod 1992;7:379–90.PubMed

45.

Stalf T, Herrero J, Mehnert C, Manolopoulos K, Lenhard A, Gips H. Influence of polarization effects in ooplasma and pronuclei on embryo quality and implantation in an IVF program. J Assist Reprod Genet 2002;19:355–62.PubMedCrossRef

46.

Palermo GD, Alikani M, Bertoli M, Colombero LT, Moy F, Cohen J, et al. Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection. Hum Reprod 1996;11:172–6.PubMed

47.

Alikani M, Palermo G, Adler A, Bertoli M, Blake M, Cohen J. Intracytoplasmic sperm njection in dysmorphic human oocytes. Zygote 1995;3:283–8.PubMedCrossRef

48.

Shen Y, Stalf T, Mehnert C, De Santis L, Cino I, Tinneberg HR, et al. Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod Biomed Online 2006;12:737–51.PubMedCrossRef

49.

Meriano JS, Alexis J, Visram-Zaver S, Cruz M, Casper RF. Tracking of oocyte dysmorphisms for ICSI patients may prove relevant to the outcome in subsequent patient cycles. Hum Reprod 2001;16:2118–23.PubMedCrossRef

50.

Balaban B, Urman B. Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online 2006;12:608–15.PubMedCrossRef

51.

De Placido G, Wilding M, Strina I, Alviggi E, Alviggi C, Mollo A, et al. High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate. Hum Reprod 2002;17:2402–9.PubMedCrossRef

52.

Van Blerkom J. Mitochondria in human oogenesis and preimplantation embryogenesis: engines of metabolism, ionic regulation and developmental competence. Reproduction 2004;128:269–80.PubMedCrossRef

53.

Wilding M, Dale B, Marino M, di Matteo L, Alviggi C, Pisaturo ML, et al. Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos. Hum Reprod 2001;16:909–17.PubMedCrossRef

Title: An oocyte score for use in assisted reproduction
Authors: Martin Wilding
Loredana Di Matteo
Sonia D’Andretti
Nadia Montanaro
Clemente Capobianco
Brian Dale
Publication date: 01-08-2007
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2007
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-007-9143-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

An oocyte score for use in assisted reproduction

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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