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Inflammopharmacology

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01-08-2019 | Original Article

Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB

Authors: Peng Zou, Hong-Ming Ji, Jian-Wei Zhao, Xin-Min Ding, Zi-Gang Zhen, Xuan Zhang, Xiao-Qi Nie, Li-Xiong Xue

Published in: Inflammopharmacology | Issue 4/2019

Abstract

Background

The study was conducted to scrutinize the outcome of isoliquiritigenin (ISL) against cerebral injury in septic mice.

Methods

The sepsis was introduced using cecal ligation and puncture (CLP) method in experimental mice. The effect of ISL was quantified using the content of brain water and blood brain barrier (BBB) permeability. The effect on the levels of myeloperoxidase (MPO), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) in brain homogenates was also determined. The effect of ISL on the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in serum was also estimated. The levels of various inflammatory biomarkers (COX-2 and PGE2) were also studied. The expression of NF-κB signalling cascade and inducible nitric oxide synthase (iNOS) was estimated by Western blot.

Results

Compared with CLP group, the brain water content was found to be reduced significantly together with the enhanced BBB integrity in ISL treated group. The level of MDA was reduced together with enhanced level of SOD and GSH in the ISL treated group. The levels of TNF-α, IL-1β, and IL-6 were also found to be modulated in ISL group. The level of COX-2 and PGE2 was reduced to near normal after ISL administration together with increase in the IκBα expression and reduction of p65 and p-p65 expression in a concentration-dependent manner. The expression of iNOS was also found to be reduced in ISL group.

Conclusion

These results demonstrate that ISL causes protection of CLP-induced sepsis in experimental mice via multiple pathways.

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Title: Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB
Authors: Peng Zou
Hong-Ming Ji
Jian-Wei Zhao
Xin-Min Ding
Zi-Gang Zhen
Xuan Zhang
Xiao-Qi Nie
Li-Xiong Xue
Publication date: 01-08-2019
Publisher: Springer International Publishing
Published in: Inflammopharmacology / Issue 4/2019
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-0503-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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