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01-10-2016 | Original Article

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Authors: Asha Rani, Subhadeep Roy, Manjari Singh, Uma Devi, Rajnish K. Yadav, Swetlana Gautam, Jitendra K. Rawat, Mohd. Nazam Ansari, Abdulaziz S. Saeedan, Anand Prakash, Gaurav Kaithwas

Published in: Inflammopharmacology | Issue 5/2016

Abstract

This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.

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Title: α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats
Authors: Asha Rani
Subhadeep Roy
Manjari Singh
Uma Devi
Rajnish K. Yadav
Swetlana Gautam
Jitendra K. Rawat
Mohd. Nazam Ansari
Abdulaziz S. Saeedan
Anand Prakash
Gaurav Kaithwas
Publication date: 01-10-2016
Publisher: Springer International Publishing
Published in: Inflammopharmacology / Issue 5/2016
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-016-0280-5

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α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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