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Inflammation
Published in: Inflammation 5/2009

01-10-2009

Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration

Authors: Francesca Porta, Hendrik Bracht, Christian Weikert, Mario Beck, Jukka Takala, Sebastian Brandt, Luzius B. Hiltebrand, Stephan M. Jakob

Published in: Inflammation | Issue 5/2009

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Abstract

Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
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Metadata
Title
Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration
Authors
Francesca Porta
Hendrik Bracht
Christian Weikert
Mario Beck
Jukka Takala
Sebastian Brandt
Luzius B. Hiltebrand
Stephan M. Jakob
Publication date
01-10-2009
Publisher
Springer US
Published in
Inflammation / Issue 5/2009
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-009-9138-y

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