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01-09-2012 | Original Article

Microsatellite instability testing in Korean patients with colorectal cancer

Authors: Jung Ryul Oh, Duck-Woo Kim, Hye Seung Lee, Hee Eun Lee, Sung Min Lee, Je-Ho Jang, Sung-Bum Kang, Ja-Lok Ku, Seung-Yong Jeong, Jae-Gahb Park

Published in: Familial Cancer | Issue 3/2012

Abstract

Microsatellite instability (MSI) testing is useful for identifying patients with hereditary nonpolyposis colorectal cancer and detecting sporadic colorectal cancer that develops through replication error pathways. A pentaplex panel is recommended by the National Cancer Institute for MSI testing, but simplified mononucleotide panels and immunohistochemistry of mismatch repair proteins are widely employed for convenience. This study was to evaluate the MSI status of colorectal cancer in Korean patients. This study included 1,435 patients with colorectal adenocarcinoma subjected to surgical resection. The pentaplex Bethesda panel was used for MSI testing. Seventy nine (5.5 %) carcinomas were classified as MSI-high (MSI-H) and 95 (6.6 %) as MSI-low (MSI-L). BAT-26 and BAT-25 were unstable in 73 and 75 of 79 MSI-H carcinomas, respectively. With the panel comprising these 2 mononucleotide markers, 72 carcinomas were diagnosed as MSI-H, compared to the Bethesda panel data (72/79, 91.1 %). In contrast, BAT-26 or BAT-25 were unstable in only 7 (7.4 %) of the 95 MSI-L tumors. In the panel with 2 dinucleotide markers, D17250 linked to p53 and D2S123 to hMSH2, detection rates were 89.9 % (71/79) for MSI-H and 80.0 % (76/95) for MSI-L carcinomas, compared to the Bethesda panel. Moreover, we compared the frequency of MSI tumor in our patients with those reported previously from Western countries. In conclusion, the frequency of MSI-H appears lower in colorectal cancer patients in Korea. A simplified panel for MSI testing with BAT-26 and BAT-25 seems not effective for the accurate evaluation of MSI status, particularly in MSI-L colorectal carcinomas, in our patients.

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Title: Microsatellite instability testing in Korean patients with colorectal cancer
Authors: Jung Ryul Oh
Duck-Woo Kim
Hye Seung Lee
Hee Eun Lee
Sung Min Lee
Je-Ho Jang
Sung-Bum Kang
Ja-Lok Ku
Seung-Yong Jeong
Jae-Gahb Park
Publication date: 01-09-2012
Publisher: Springer Netherlands
Published in: Familial Cancer / Issue 3/2012
Print ISSN: 1389-9600
Electronic ISSN: 1573-7292
DOI: https://doi.org/10.1007/s10689-012-9536-4

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