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Open Access 01-11-2009 | Methods

Determinants of DNA yield and purity collected with buccal cell samples

Authors: D. B. M. A. van Wieren-de Wijer, A. H. Maitland-van der Zee, A. de Boer, S. V. Belitser, A. A. Kroon, P. W. de Leeuw, P. Schiffers, R. G. J. H. Janssen, C. M. van Duijn, B. H. C. H. Stricker, O. H. Klungel

Published in: European Journal of Epidemiology | Issue 11/2009

Abstract

Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 μg (median 54.3 μg; mean 82.2 μg ± SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 μg) than those from women (median 44.2 μg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.

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Title: Determinants of DNA yield and purity collected with buccal cell samples
Authors: D. B. M. A. van Wieren-de Wijer
A. H. Maitland-van der Zee
A. de Boer
S. V. Belitser
A. A. Kroon
P. W. de Leeuw
P. Schiffers
R. G. J. H. Janssen
C. M. van Duijn
B. H. C. H. Stricker
O. H. Klungel
Publication date: 01-11-2009
Publisher: Springer Netherlands
Published in: European Journal of Epidemiology / Issue 11/2009
Print ISSN: 0393-2990
Electronic ISSN: 1573-7284
DOI: https://doi.org/10.1007/s10654-009-9388-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Determinants of DNA yield and purity collected with buccal cell samples

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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