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01-10-2009 | Original Article

Pseudomonas aeruginosa: Mannose Sensitive Hemagglutinin Inhibits the Growth of Human Hepatocarcinoma Cells via Mannose-Mediated Apoptosis

Authors: Zhenyuan Cao, Lijun Shi, Ying Li, Jinghua Wang, Dandan Wang, Guangyou Wang, Bo Sun, Lili Mu, Mingfei Yang, Hulun Li

Published in: Digestive Diseases and Sciences | Issue 10/2009

Abstract

A vaccine derived from the outer membrane proteins of the Gram-negative bacterium Pseudomonas aeruginosa has been shown to have immune modulatory properties. An inactivated mutant strain of P. aeruginosa with mannose sensitive hemagglutinin fimbria (PA-MSHA) has been used for adjuvant therapy for malignant cancer. In this study, the growth of human hepatocellular carcinoma Hep G2 and BEL-7402 cells is inhibited by PA-MSHA, but not by mannose-cleaved PA-MSHA. PA-MSHA-treated cells arrested in the S phase of the cell cycle and underwent apoptosis. We hypothesize that apoptosis induced by treatment of Hep G2 and BEL-7402 cells with PA-MSHA is mediated by the mannose residues of PA-MSHA and is propagated through the extrinsic apoptosis pathway directly through caspase-8. These data provide mechanistic details for the potential application of PA-MSHA-based treatment of hepatocellular carcinoma.

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Title: Pseudomonas aeruginosa: Mannose Sensitive Hemagglutinin Inhibits the Growth of Human Hepatocarcinoma Cells via Mannose-Mediated Apoptosis
Authors: Zhenyuan Cao
Lijun Shi
Ying Li
Jinghua Wang
Dandan Wang
Guangyou Wang
Bo Sun
Lili Mu
Mingfei Yang
Hulun Li
Publication date: 01-10-2009
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 10/2009
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0603-5

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