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Journal of Medical Ultrasonics

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01-01-2015 | Original Article

Combination of ultrasound and newly synthesized magnetic nanocapsules affects the temperature profile of CT26 tumors in BALB/c mice

Authors: Ali Shakeri-Zadeh, Samideh Khoei, Sepideh Khoee, Ali Mohammad Sharifi, Mohammad-Bagher Shiran

Published in: Journal of Medical Ultrasonics | Issue 1/2015

Abstract

Purpose

To investigate the effects of a combination of 3-MHz ultrasound waves with a new magnetic nanocapsule containing 5-fluorouracil (5-Fu) on the temperature profile of a mouse colon tumor (CT26) in BALB/c mice.

Methods

Firstly, 5-Fu-loaded magnetic nanocapsules were synthesized using a multiple emulsion solvent evaporation procedure. Magnetic resonance imaging (MRI) was performed to evaluate the efficiency of nanocapsule localization in the tumor during magnetic drug targeting (MDT). Tumors were separately exposed to 3-MHz ultrasound waves at the intensities of 0.1, 0.3, 0.5, and 1 W/cm² for 10 min in the absence and presence of nanocapsules. The temperature of the tumor was recorded at 1-min intervals.

Results

The effective diameter of the nanocapsules was approximately 70 nm, and it was demonstrated that magnetic nanoparticles were well dispersed inside the nanocapsules. MRI confirmed that the magnetic nanocapsules were successfully targeted to the tumor after accomplishing MDT. Temperature change due to sonication of the tumor was strongly intensity dependent. Moreover, temperature–time curves revealed that the magnetic nanocapsules significantly affected the temperature rise profile of a sonicated tumor.

Conclusion

Data presented in this study would be helpful to develop an ultrasound-mediated MDT procedure so that temperature changes of the tumor and its surrounding normal tissues may be controllable.

Konofagou E. Mechanism, monitoring, and drug delivery of the ultrasound-induced blood-brain barrier opening. J Acoust Soc Am. 2013;134:4089.CrossRef

Rapoport NY, Kennedy AM, Shea JE, et al. Controlled and targeted tumor chemotherapy by ultrasound-activated nanoemulsions/microbubbles. J Control Release. 2009;138:268–76.PubMedCentralPubMedCrossRef

Gao ZG, Fain HD, Rapoport N. Controlled and targeted tumor chemotherapy by micellar-encapsulated drug and ultrasound. J Control Release. 2005;102:203–22.PubMedCrossRef

Khoee S, Rahmatolahzadeh R. Synthesis and characterization of pH-responsive and folated nanoparticles based on self-assembled brush-like PLGA/PEG/AEMA copolymer with targeted cancer therapy properties: a comprehensive kinetic study. Eur J Med Chem. 2012;50:416–27.PubMedCrossRef

Khoee S, Hossainzadeh MT. Effect of O/S/W process parameters on 17beta-EV loaded nanoparticles properties. Colloids Surf B Biointerfaces. 2010;75:133–40.PubMedCrossRef

Shakeri-Zadeh A, Khoei S, Khoee S, et al. Targeted, monitored, and controlled chemotherapy: a multimodal nanotechnology-based approach against cancer. ISRN Nanotechnol. 2013. Article ID 629510.

Wu J, Akaike T, Hayashida K, et al. Enhanced vascular permeability in solid tumor involving peroxynitrite and matrix metalloproteinases. Jpn J Cancer Res. 2001;92:439–51.PubMedCrossRef

Gong L, Zhang Y, Su L. Target distribution of magnetic albumin nanoparticles containing adriamycin in transplanted rat liver cancer model. Hepatobiliary Pancreat Dis Int. 2004;3:365.PubMed

Ashjari M, Khoee S, Mahdavian AR. A multiple emulsion method for loading 5-fluorouracil into a magnetite-loaded nanocapsule: a physicochemical investigation. Polym Int. 2012;61:850.CrossRef

10.

Mohammadi SD, Khoei S, Mahdavi SR. The combination effect of poly(lactic-co-glycolic acid) coated iron oxide nanoparticles as 5-fluorouracil carrier and X-ray on the level of DNA damages in the DU 145 human prostate carcinoma cell line. J Bionanosci. 2012;6:23–7.CrossRef

11.

Chen PY, Liu HL, Hua MY, et al. Novel magnetic/ultrasound focusing system enhances nanoparticle drug delivery for glioma treatment. Neuro Oncol. 2010;12:1050–60.PubMedCentralPubMedCrossRef

12.

Fan CH, Ting CY, Lin HJ, et al. SPIO-conjugated, doxorubicin-loaded microbubbles for concurrent MRI and focused-ultrasound enhanced brain-tumor drug delivery. Biomaterials. 2013;34:3706–15.PubMedCrossRef

13.

Liu HL, Hua MY, Yang HW, et al. Magnetic resonance monitoring of focused ultrasound/magnetic nanoparticle targeting delivery of therapeutic agents to the brain. Proc Natl Acad Sci USA. 2010;107:15205–10.PubMedCentralPubMedCrossRef

14.

Frenkel V. Ultrasound mediated delivery of drugs and genes to solid tumors. Adv Drug Deliv Rev. 2008;60:1193–208.PubMedCentralPubMedCrossRef

15.

Chan AK, Myrer JW, Measom GJ, et al. Temperature changes in human patellar tendon in response to therapeutic ultrasound. J Athl Train. 1998;33:130–5.PubMedCentralPubMed

16.

Hayes BT, Merrick MA, Sandrey MA, et al. Three-MHz ultrasound heats deeper into the tissues than originally theorized. J Athl Train. 2004;39:230–4.PubMedCentralPubMed

17.

Quan M, Shiran MB, Watmough DJ. Effect of wave diffraction on the measurement of ultrasonic power output using the radiation force method with a plane target angled at 45. Meas Sci Technol. 1992;3:222–7.CrossRef

18.

Kossoff G. Balance technique for the measurement of very low ultrasonic power output. J Acoust Soc Am. 2005;38:880–1.CrossRef

19.

Watmough DJ, Quan KM, Shiran MB. The Sarvazyan method of mapping ultrasound fields. Ultrasound Instrum IEE Colloq Lond. 1990;4:1–3.

20.

Wang JM, Xiao BL, Zheng JW, et al. Effect of targeted magnetic nanoparticles containing 5-FU on expression of bcl-2, bax and caspase 3 in nude mice with transplanted human liver cancer. World J Gastroenterol. 2007;13:3171.PubMed

21.

Souchon R, Bouchoux G, Maciejko E, et al. Monitoring the formation of thermal lesions with heat-induced echo-strain imaging: a feasibility study. Ultrasound Med Biol. 2005;31:251–9.PubMedCrossRef

22.

Shiran MB, Motevalian M, Ravanfar R, et al. The effect of bubble surface charge on phonophoresis: implication in transdermal piroxicam delivery. Iran J Pharmacol Ther. 2008;7:15–9.

23.

Dehkordi F, Shakeri-Zadeh A, Khoei S, et al. Thermal distribution of ultrasound waves in prostate tumor: comparison of computational modeling with in vivo experiments. ISRN Biomath. 2013: Article ID 428659.

24.

Curiel L, Chavrier F, Gignoux B, et al. Experimental evaluation of lesion prediction modelling in the presence of cavitation bubbles: intended for high-intensity focused ultrasound prostate treatment. Med Biol Eng Comp. 2004;42:44–54.CrossRef

25.

Draper DO, Castel JC, Caster D. Rate of temperature increase in human muscle during 1 MHz and 3 MHz continuous ultrasound. J Orthop Sports Phys Ther. 1995;22:142–50.PubMedCrossRef

Title: Combination of ultrasound and newly synthesized magnetic nanocapsules affects the temperature profile of CT26 tumors in BALB/c mice
Authors: Ali Shakeri-Zadeh
Samideh Khoei
Sepideh Khoee
Ali Mohammad Sharifi
Mohammad-Bagher Shiran
Publication date: 01-01-2015
Publisher: Springer Japan
Published in: Journal of Medical Ultrasonics / Issue 1/2015
Print ISSN: 1346-4523
Electronic ISSN: 1613-2254
DOI: https://doi.org/10.1007/s10396-014-0558-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Combination of ultrasound and newly synthesized magnetic nanocapsules affects the temperature profile of CT26 tumors in BALB/c mice

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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