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Japanese Journal of Ophthalmology

Published in:

01-05-2012 | Laboratory Investigation

Histopathologic analysis of the internal limiting membrane surgically peeled from eyes with diffuse diabetic macular edema

Authors: Kaoru Tamura, Toshiyuki Yokoyama, Nobuyuki Ebihara, Akira Murakami

Published in: Japanese Journal of Ophthalmology | Issue 3/2012

Abstract

Purpose

To investigate the pathogenesis of diffuse diabetic maculae edema (DME).

Methods

Internal limiting membrane (ILM) specimens were surgically peeled from 16 eyes of 15 patients with diffuse DME (diabetic retinopathy (DR) group) and from 12 eyes of 12 patients without diabetes (non-DR group). The specimens were then examined by light microscopy (LM), transmission electron microscopy (TEM), and immunohistochemistry.

Results

Examination by LM revealed numerous cells on the inner surface of the ILM specimens in the DR-group, among which five different cell types (glial cell, fibroblast-like cell, macrophages, neutrophils, and lymphocytes) were confirmed. Examination by TEM revealed that the thickness of the peeled ILMs in the DR group was significantly greater, and four types of cellular element (glial, fibroblast-like, macrophages, and lymphocytes) were confirmed on the surface of the vitreous side of the specimens. The existence of glial cells and macrophages was confirmed by immunohistochemistry.

Conclusions

In diffuse DME, the ILM is thickened and a variety of cellular elements, especially numerous kinds of inflammatory cell, adhere to the inner surface of the ILM. To attenuate diffuse DME, vitrectomy combined with ILM peeling to remove the inflammatory cells and the physical barrier might be effective.

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Title: Histopathologic analysis of the internal limiting membrane surgically peeled from eyes with diffuse diabetic macular edema
Authors: Kaoru Tamura
Toshiyuki Yokoyama
Nobuyuki Ebihara
Akira Murakami
Publication date: 01-05-2012
Publisher: Springer Japan
Published in: Japanese Journal of Ophthalmology / Issue 3/2012
Print ISSN: 0021-5155
Electronic ISSN: 1613-2246
DOI: https://doi.org/10.1007/s10384-012-0130-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Histopathologic analysis of the internal limiting membrane surgically peeled from eyes with diffuse diabetic macular edema

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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