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Journal of the Association for Research in Otolaryngology

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01-02-2014 | Research Article

Ouabain-Induced Cochlear Nerve Degeneration: Synaptic Loss and Plasticity in a Mouse Model of Auditory Neuropathy

Authors: Yasheng Yuan, Fuxin Shi, Yanbo Yin, Mingjie Tong, Hainan Lang, Daniel B. Polley, M. Charles Liberman, Albert S.B. Edge

Published in: Journal of the Association for Research in Otolaryngology | Issue 1/2014

Abstract

Ouabain application to the round window can selectively destroy type-I spiral ganglion cells, producing an animal model of auditory neuropathy. To assess the long-term effects of this deafferentation on synaptic organization in the organ of Corti and cochlear nucleus, and to ask whether surviving cochlear neurons show any post-injury plasticity in the adult, we quantified the peripheral and central synapses of type-I neurons at posttreatment times ranging from 1 to 3 months. Measures of normal DPOAEs and greatly reduced auditory brainstem responses (ABRs) confirmed the neuropathy phenotype. Counts of presynaptic ribbons and postsynaptic glutamate receptor patches in the inner hair cell area decreased with post-exposure time, as did counts of cochlear nerve terminals in the cochlear nucleus. Although these counts provided no evidence of new synapse formation via branching from surviving neurons, the regular appearance of ectopic neurons in the inner hair cell area suggested that neurite extension is not uncommon. Correlations between pathophysiology and histopathology showed that ABR thresholds are very insensitive to even massive neural degeneration, whereas the amplitude of ABR wave 1 is a better metric of synaptic degeneration.

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Title: Ouabain-Induced Cochlear Nerve Degeneration: Synaptic Loss and Plasticity in a Mouse Model of Auditory Neuropathy
Authors: Yasheng Yuan
Fuxin Shi
Yanbo Yin
Mingjie Tong
Hainan Lang
Daniel B. Polley
M. Charles Liberman
Albert S.B. Edge
Publication date: 01-02-2014
Publisher: Springer US
Published in: Journal of the Association for Research in Otolaryngology / Issue 1/2014
Print ISSN: 1525-3961
Electronic ISSN: 1438-7573
DOI: https://doi.org/10.1007/s10162-013-0419-7

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Ouabain-Induced Cochlear Nerve Degeneration: Synaptic Loss and Plasticity in a Mouse Model of Auditory Neuropathy

Abstract

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Springer Medicine

Abstract

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