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01-09-2019 | Cytokines | Original Article

In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts

Authors: Daíse Raquel Maldaner, Verônica Farina Azzolin, Fernanda Barbisan, Moisés Henrique Mastella, Cibele Ferreira Teixeira, Alexandre Dihel, Thiago Duarte, Neida Luiza Pellenz, Luiz Fernando Cuozzo Lemos, Carla Maria Uggeri Negretto, Ivana Beatrice Mânica da Cruz, Marta Maria Medeiros Frescura Duarte

Published in: Lasers in Medical Science | Issue 7/2019

Abstract

Skin aging is a complex biological process induced by intrinsic and extrinsic factors which is characterized by clinical and cellular changes, especially dermal fibroblasts. It is possible that, some procedures, such as low-level laser therapy (LLLT), could decelerate this process. To test this hypothesis, this study evaluated the in vitro LLLT on dermal fibroblast cell line (HFF-1) with premature senescence H₂O₂-induced. HFF-1 cells were cultured in standardized conditions, and initially H₂O₂ exposed at different concentrations. Fibroblasts were also just exposed at different LLLT (660 nm) doses. From these curves, the lowest H₂O₂ concentration that induced indicators of premature senescence and the lowest LLLT doses that triggered fibroblast proliferation were used in all assays. Cellular mortality, proliferation, and the levels of oxidative, inflammatory cytokines, apoptotic markers, and of two growth signaling molecules (FGF-1 and KGF) were compared among treatments. The H₂O₂ at 50 μM concentration induced some fibroblast senescence markers and for LLLT, the best dose for treatment was 4 J (p < 0.001). The interaction between H₂O₂ at 50 μM and LLLT at 4 J showed partially reversion of the higher levels of DNA oxidation, CASP 3, CASP 8, IL-1B, IL-6, and INFy induced by H₂O₂ exposure. LLLT also trigger increase of IL-10 anti-inflammatory cytokine, FGF-1 and KGF levels. Cellular proliferation was also improved when fibroblasts treated with H₂O₂ were exposed to LLLT (p < 0.001). These results suggest that in fibroblast with some senescence characteristics H₂O₂-induced, the LLLT presented an important protective and proliferative action, reverting partially or totally negative effects triggering by H₂O₂.

Lephart ED (2016) Skin aging and oxidative stress: Equol’s anti-aging effects via biochemical and molecular mechanisms. Ageing Res Rev 31:36–54CrossRefPubMed

Cole MA, Quan T, Voorhees JJ, Fisher GJ (2018) Extracellular matrix regulation of fibroblast function: redefining our perspective on skin aging. J Cell Commun Signal 12:35–43CrossRefPubMedPubMedCentral

Birch-Machin MA, Bowman A (2016) Oxidative stress and ageing. Br J Dermatol 175:26–29CrossRefPubMed

Silva SAME, Michniak-Kohn B, Leonardi GR (2017) An overview about oxidation in clinical practice of skin aging. An Bras Dermatol 92:367–374CrossRefPubMedPubMedCentral

Zachary CB (2016) Facial rejuvenation: 40^th anniversary review. Semin Cutan Med Surg 35:122–124CrossRef

Ramos FS, Maifrino LBM, Alves da Costa Aguiar S, Perez MM, Feder D, Azzalis LA et al (2018) The effects of transcutaneous low-level laser therapy on the skin healing process: an experimental model. Lasers Med Sci 33:967–976CrossRefPubMed

McFarland GA, Holliday R (1999) Further evidence for the rejuvenating effects of the dipeptide L-carnosine on culture human diploid fibroblasts. Exp Gerontol 34:35–45CrossRefPubMed

Merrell JG, McLaughlin SW, Tie L, Laurencin CT, Chen AF, Nair LS (2009) Curcumin-loaded poly(epsilon-caprolactone) nanofibres: diabetic wound dressing with anti-oxidant and anti-inflammatory properties. Clin Exp Pharmacol Physiol 36:1149–1156CrossRefPubMedPubMedCentral

Delgado L, Fernandes I, Gonzaléz-Manzano S, de Freitas V, Mateus N, Santos-Buelga C (2014) Anti-proliferative effects of quercetin and catechin metabolites. Food Funct 5:797–803CrossRefPubMed

10.

Zdanov S, Remacle J, Toussaint O (2006) Establishment of H₂O₂-induced premature senescence in human fibroblastsconcomitant with increased cellular production of H₂O₂. Ann N Y Acad Sci. 1067:210–216CrossRefPubMed

11.

Cirillo N, Vicidomini A, McCullough M, Gambardella A, Hassona Y, Prime SS et al (2015) A hyaluronic acid-based compound inhibits fibroblast senescence induced by oxidative stress in vitro and prevents oral mucositis in vivo. J Cell Physiol 230:1421–1429CrossRefPubMed

12.

Barbisan F, Azzolin VF, Ribeiro EE, Duarte MMMF, da Cruz IBM (2017) The in vitro influence of a genetic superoxide-hydrogen peroxide imbalance on immunosenescence. Rejuvenation Res 20:334–345CrossRefPubMed

13.

Costa F, Barbisan F, Assmann CE, Araújo NKF, de Oliveira AR, Signori JP et al (2017) Seminal cell-free DNA levels measured by picogreen fluorochrome are associated with sperm fertility criteria. Zygote 25:111–119CrossRefPubMed

14.

Azzolin VF, Cadoná FC, Machado AK, Berto MD, Barbisan F, Dornelles EB et al (2016) Superoxide-hydrogen peroxide imbalance interferes with colorectal cancer cells viability, proliferation and oxaliplatin response. Toxicol in Vitro 32:8–15CrossRefPubMed

15.

Toussaint O, Medrano EE, von Zglinicki T (2000) Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes. Exp Gerontol 35:927–945CrossRefPubMed

16.

Marthandan S, Menzel U, Priebe S, Groth M, Guthke R, Platzer M (2016) Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34CrossRefPubMedPubMedCentral

17.

Ortiz-Espín A, Morel E, Juarranz Á, Guerrero A, González S, Jiménez A et al (2017) An extract from the plant Deschampsia antarctica protects fibroblasts from senescence induced by hydrogen peroxide. Oxidative Med Cell Longev 2017:2694945CrossRef

18.

Kuilman TC, Michaloglou C, Mooi WJ, Peeper DS (2010) The essence of senescence. Genes Dev 24:2463–2479CrossRefPubMedPubMedCentral

19.

Campisi J (2011) Cellular senescence: putting the paradoxes in perspective. Curr Opin Genet Dev 21:107–112CrossRefPubMed

20.

Turner JD, Naylor AJ, Buckley C, Filer A, Tak PP (2018) Fibroblasts and osteoblasts in inflammation and bone damage. Adv Exp Med Biol 1060:37–54CrossRefPubMed

21.

Yun IG, Ahn SH, Yoon WJ, Kim CS, Lim YK, Kook JK (2018) Litsea japonica leaf extract suppresses Proinflammatory cytokine production in periodontal ligament fibroblasts stimulated with Oral pathogenic bacteria or interleukin-1β. Int J Mol Sci 23(19)

22.

Shirato K, Koda T, Takanari J, Sakurai T, Ogasawara J, Imaizumi K et al (2018) Anti-inflammatory effect of ETAS®50 by inhibiting nuclear factor-κβ p65 nuclear import in ultraviolet-B-irradiated normal human dermal fibroblasts. Evid Based Complement Alternat Med 2018:5072986CrossRefPubMedPubMedCentral

23.

Verma A, Stellacci F (2010) Effect of surface on nanoparticle-cell interactions. Small 6:12–21CrossRefPubMed

24.

Bayat M, Virdi A, Jalalifirouzkouhi R, Rezaei F (2018) Comparison of effects of LLLT and LIPUS on fracture healing in animal models and patients: a systematic review. Prog Biophys Mol Biol 132:3–22CrossRefPubMed

25.

Migliario M, Sabbatini M, Mortellaro C, Renò F (2018) Near infrared low level laser therapy and cell proliferation: the emerging role of redox sensitive signal transduction pathways. J Biophotonics 11(11):e201800025CrossRefPubMed

26.

Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C et al (2017) Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H₂O₂-induced premature senescence through SIRT1. Am J Transl Res 9:4492–4501PubMedPubMedCentral

27.

Devi S, Kumar N, Kapila S, Mada SB, Reddi S, Vij R et al (2017) Buffalo casein derived peptide can alleviates H₂O₂ induced cellular damage and necrosis in fibroblast cells. Exp Toxicol Pathol 69:485–495CrossRefPubMed

28.

Huang C, Qian SL, Sun LY, Cheng B (2016) Light-emitting diode irradiation (640 nm) regulates keratinocyte migration and cytoskeletal reorganization via hypoxia-inducible factor-1α. Photomed Laser Surg 34:313–320CrossRefPubMed

29.

Szezerbaty SKF, de Oliveira RF, Pires-Oliveira DAA, Soares CP, Sartori D, Poli-Frederico RC (2018) The effect of low-level laser therapy (660 nm) on the gene expression involved in tissue repair. Lasers Med Sci 33:315–321CrossRefPubMed

30.

Silveira PC, Scheffer Dda L, Glaser V, Remor AP, Pinho RA, Aguiar Junior AS, Latini A (2016) Low-level laser therapy attenuates the acute inflammatory response induced by muscle traumatic injury. Free Radic Res 50:503–513CrossRefPubMed

31.

Da Ré Guerra F, Vieira CP, Oliveira LP, Marques PP, dos Santos Almeida M, Pimentel ER (2016) Low-level laser therapy modulates pro-inflammatory cytokines after partial tenotomy. Lasers Med Sci 31:759–766CrossRefPubMed

32.

Żerańska J, Pasikowska M, Szczepanik B, Mlosek K, Malinowska S, Dębowska RM et al (2016) A study of the activity and effectiveness of recombinant fibroblast growth factor (Q40P/S47I/H93G rFGF-1) in anti-aging treatment. Postepy Dermatol Alergol 33:28–36CrossRefPubMedPubMedCentral

33.

Denadai AS, Aydos RD, Silva IS, Olmedo L, de Senna Cardoso BM, da Silva BAK et al (2017) Acute effects of low-level laser therapy (660 nm) on oxidative stress levels in diabetic rats with skin wounds. J Exp Ther Oncol 11:85–89PubMed

34.

Pansani TN, Basso FG, Turrioni AP, Soares DG, Hebling J, de Souza Costa CA (2017) Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals. Lasers Med Sci 32:45–52CrossRefPubMed

35.

Canady J, Arndt S, Karrer S, Bosserhoff AK (2013) Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis. J Invest Dermatol 133:647–657CrossRefPubMed

36.

Migliario M, Pittarella P, Fanuli M, Rizzi M, Renò F (2014) Laser-induced osteoblast proliferation is mediated by ROS production. Lasers Med Sci 29:1463–1467CrossRefPubMed

37.

Kreslavski VD, Fomina IR, Carpentier DALR, Kuznetsov VV, Allakhverdiev SI (2012) Red and near infra-red signaling: hypothesis and perspectives. J Photochem Photobiol C: Photochem Rev 13:190–203CrossRef

38.

Rathnakar B, Rao BSS, Prabhu V, Chandra S, Mahato KK (2018) Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice. Lasers Med Sci 33(4):699–707CrossRefPubMed

Title: In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts
Authors: Daíse Raquel Maldaner
Verônica Farina Azzolin
Fernanda Barbisan
Moisés Henrique Mastella
Cibele Ferreira Teixeira
Alexandre Dihel
Thiago Duarte
Neida Luiza Pellenz
Luiz Fernando Cuozzo Lemos
Carla Maria Uggeri Negretto
Ivana Beatrice Mânica da Cruz
Marta Maria Medeiros Frescura Duarte
Publication date: 01-09-2019
Publisher: Springer London
Keywords: Cytokines
Laser
Published in: Lasers in Medical Science / Issue 7/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02728-1

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In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts

Abstract

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Springer Medicine

Abstract

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