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01-09-2018 | Original Article

LED phototherapy in full-thickness burns induced by CO₂ laser in rats skin

Authors: Milene da Silva Melo, Leandro Procópio Alves, Adriana Barrinha Fernandes, Henrique Cunha Carvalho, Carlos José de Lima, Egberto Munin, Mônica Fernandes Gomes, Miguel Angel Castillo Salgado, Renato Amaro Zângaro

Published in: Lasers in Medical Science | Issue 7/2018

Abstract

Many studies have been conducted on the treatment of burns because they are important in morbidity and mortality. These studies are mainly focused on improving care and quality of life of patients. The aim of this study was evaluate the LED phototherapy effects in rats skin full-thickness burns induced by CO₂ laser. The animals were divided in NT group that did not received any treatment and LED group that received LED irradiation at 685 nm, 220 mW, and 4.5 J/cm² during 40 s by burned area. Biopsies were obtained after 7, 14, and 21 days of treatment and submitted to histological and immunohistochemical analysis. The LED phototherapy shows anti-inflammatory effects, improves angiogenesis, and stimulates the migration and proliferation of fibroblasts. The T CD8+ lymphocytes were more common in burned areas compared to T CD4+ lymphocytes since statistically significant differences were observed in the LED group compared to the NT group after 7 days of treatment. These results showed that LED phototherapy performs positive influence in full-thickness burns repair from the healing process modulated by cellular immune response. The obtained results allowed inferring that burns exhibit a characteristic cell immune response and this cannot be extrapolated to other wounds such as incision and wounds induced by punch, among others.

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Title: LED phototherapy in full-thickness burns induced by CO2 laser in rats skin
Authors: Milene da Silva Melo
Leandro Procópio Alves
Adriana Barrinha Fernandes
Henrique Cunha Carvalho
Carlos José de Lima
Egberto Munin
Mônica Fernandes Gomes
Miguel Angel Castillo Salgado
Renato Amaro Zângaro
Publication date: 01-09-2018
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 7/2018
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2515-8

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LED phototherapy in full-thickness burns induced by CO₂ laser in rats skin

Abstract

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Abstract

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