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01-02-2016 | Original Article

Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells

Authors: Samereh Dehghani Soltani, Abdolreza Babaee, Mohammad Shojaei, Parvin Salehinejad, Fatemeh Seyedi, Mahshid JalalKamali, Seyed Noureddin Nematollahi-Mahani

Published in: Lasers in Medical Science | Issue 2/2016

Abstract

Light-emitting diodes (LED) have recently been introduced as a potential factor for proliferation of various cell types in vitro. Nowadays, stem cells are widely used in regenerative medicine. Human umbilical cord matrix-derived mesenchymal (hUCM) cells can be more easily isolated and cultured than adult mesenchymal stem cells. The aim of this study was to evaluate the effect of red and green lights produced by LED on the proliferation of hUCM cells. hUCM cells were isolated from the umbilical cord, and light irradiation was applied at radiation energies of 0.318, 0.636, 0.954, 1.59, 3.18, 6.36, 9.54, and 12.72 J/cm². Irradiation of the hUCM cells shows a significant (p < 0.05) increase in cell number as compared to controls after 40 h. In addition, cell proliferation on days 7, 14, and 21 in irradiated groups were significantly (p < 0.001) higher than that in the non-irradiated groups. The present study clearly demonstrates the ability of red and green lights irradiation to promote proliferation of hUCM cells in vitro. The energy applied to the cells through LED irradiation is an effective factor with paradoxical alterations. Green light inserted a much profound effect at special dosages than red light.

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Title: Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells
Authors: Samereh Dehghani Soltani
Abdolreza Babaee
Mohammad Shojaei
Parvin Salehinejad
Fatemeh Seyedi
Mahshid JalalKamali
Seyed Noureddin Nematollahi-Mahani
Publication date: 01-02-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1846-y

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Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells

Abstract

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