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Lasers in Medical Science
Published in: Lasers in Medical Science 9/2015

01-12-2015 | Original Article

The effect of ephrin-A1 on resistance to Photofrin-mediated photodynamic therapy in esophageal squamous cell carcinoma cells

Authors: Pei-Wen Yang, Tzu-Hsuan Chiang, Ching-Yueh Hsieh, Ya-Chuan Huang, Li-Fan Wong, Mien-Chie Hung, Jui-Chang Tsai, Jang-Ming Lee

Published in: Lasers in Medical Science | Issue 9/2015

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Abstract

Esophageal squamous cell carcinoma (ESCC), the most prevalent cell type of esophageal cancer, remains a dismal disease with poor prognosis. Photodynamic therapy (PDT) is a minimally invasive treatment option for early esophageal cancer. To explore possible factors involved in resistance to PDT in esophageal cancer cells, we selected PDT-resistant subcell lines by repeated treatment of CE48T/VGH (CE48T) ESCC cells with Photofrin-PDT and then analyzed the global gene modulations in the PDT-resistant cells by whole-genome microarray. More than 700 genes reached a fold change greater than 1.5 in each of the PDT-resistant cells compared to parental cells. Among these genes, both tumor necrosis factor (TNF) and EFNA1 genes were significantly upregulated in resistant cell lines. However, they were significantly downregulated in Photofrin-PDT-treated cells compared to untreated cells. The observations made in the microarray analysis were further confirmed by quantitative PCR. We observed that recombinant tumor necrosis factor alpha (TNF-α) activated the gene expression of EFNA1 at both the messenger RNA (mRNA) level and the protein level in CE48T cells. Functional analysis showed that when incubated with oligomeric and monomeric ephrin-A1 simultaneously, ESCC cells became significantly resistant to Photofrin-PDT. Functional analysis further suggested that transmembrane and soluble ephrin-A1 may cooperate to enhance resistance to Photofrin-PDT in ESCC cells.
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Metadata
Title
The effect of ephrin-A1 on resistance to Photofrin-mediated photodynamic therapy in esophageal squamous cell carcinoma cells
Authors
Pei-Wen Yang
Tzu-Hsuan Chiang
Ching-Yueh Hsieh
Ya-Chuan Huang
Li-Fan Wong
Mien-Chie Hung
Jui-Chang Tsai
Jang-Ming Lee
Publication date
01-12-2015
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 9/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-015-1812-8

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