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01-07-2014 | Original Article

Evaluating the effect of low-level laser therapy on healing of tentomized Achilles tendon in streptozotocin-induced diabetic rats by light microscopical and gene expression examinations

Authors: Morteza Aliodoust, Mohammad Bayat, Mohammad Reza Jalili, Zainalabedin Sharifian, Masoomeh Dadpay, Mohammad Akbari, Mehrnoush Bayat, Amir Khoshvaghti, Homa Bayat

Published in: Lasers in Medical Science | Issue 4/2014

Abstract

Tendon healing is impaired in individuals diagnosed with diabetes mellitus (DM). According to research, there is considerable improvement in the healing of surgically tenotomized Achilles tendons following low-level laser therapy (LLLT) in non-diabetic, healthy animals. This study uses light microscopic (LM) and semi-quantitative reverse transcription PCR (RT-PCR) analyses to evaluate the ability of LLLT in healing Achilles tendons from streptozotocin-induced diabetic (STZ-D) rats. A total of 88 rats were randomly divided into two groups, non-diabetic and diabetic. DM was induced in the rats by injections of STZ. The right Achilles tendons of all rats were tenotomized 1 month after administration of STZ. Laser-treated rats were treated with a helium–neon (He–Ne) laser that had a 632.8-nm wavelength and 7.2-mW average power. Experimental group rats received a daily dose of 0.014 J (energy density, 2.9 J/cm²). Control rats did not receive LLLT. Animals were sacrificed on days 5, 10, and 15 post-operatively for semi-quantitative LM and semi-quantitative RT-PCR examinations of transforming growth factor-beta1 (TGF-β1) gene expression. The chi-square test showed that LLLT significantly reduced inflammation in non-diabetic rats compared with their non-diabetic controls (p = 0.02). LLLT significantly decreased inflammation in diabetic rats on days 5 (p = 0.03) and 10 (p = 0.02) compared to the corresponding control diabetic rats. According to the student’s t test, LLLT significantly increased TGF-β1 gene expression in healthy (p = 0.000) and diabetic (p = 0.000) rats compared to their relevant controls. The He–Ne laser was effective in altering the inflammatory reaction and increasing TGF-β1 gene production.

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Title: Evaluating the effect of low-level laser therapy on healing of tentomized Achilles tendon in streptozotocin-induced diabetic rats by light microscopical and gene expression examinations
Authors: Morteza Aliodoust
Mohammad Bayat
Mohammad Reza Jalili
Zainalabedin Sharifian
Masoomeh Dadpay
Mohammad Akbari
Mehrnoush Bayat
Amir Khoshvaghti
Homa Bayat
Publication date: 01-07-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 4/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1561-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluating the effect of low-level laser therapy on healing of tentomized Achilles tendon in streptozotocin-induced diabetic rats by light microscopical and gene expression examinations

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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