A literature review and novel theoretical approach on the optical properties of whole blood

Optical property measurements on blood are influenced by a large variety of factors of both physical and methodological origin. The aim of this review is to list these factors of influence and to provide the reader with optical property spectra (250–2,500 nm) for whole blood that can be used in the practice of biomedical optics (tabulated in the appendix). Hereto, we perform a critical examination and selection of the available optical property spectra of blood in literature, from which we compile average spectra for the absorption coefficient (μ_a), scattering coefficient (μ_s) and scattering anisotropy (g). From this, we calculate the reduced scattering coefficient (μ_s′) and the effective attenuation coefficient (μ_eff). In the compilation of μ_a and μ_s, we incorporate the influences of absorption flattening and dependent scattering (i.e. spatial correlations between positions of red blood cells), respectively. For the influence of dependent scattering on μ_s, we present a novel, theoretically derived formula that can be used for practical rescaling of μ_s to other haematocrits. Since the measurement of the scattering properties of blood has been proven to be challenging, we apply an alternative, theoretical approach to calculate spectra for μ_s and g. Hereto, we combine Kramers–Kronig analysis with analytical scattering theory, extended with Percus–Yevick structure factors that take into account the effect of dependent scattering in whole blood. We argue that our calculated spectra may provide a better estimation for μ_s and g (and hence μ_s′ and μ_eff) than the compiled spectra from literature for wavelengths between 300 and 600 nm.