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Lasers in Medical Science
Published in: Lasers in Medical Science 5/2013

01-09-2013 | Original Article

LLLT improves tendon healing through increase of MMP activity and collagen synthesis

Authors: Flávia Da Ré Guerra, Cristiano Pedrozo Vieira, Marcos Santos Almeida, Letícia Prado Oliveira, Andrea Aparecida de Aro, Edson Rosa Pimentel

Published in: Lasers in Medical Science | Issue 5/2013

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Abstract

The Achilles tendon has a high incidence of rupture, and the healing process leads to a disorganized extracellular matrix (ECM) with a high rate of injury recurrence. To evaluate the effects of different conditions of low-level laser (LLL) application on partially tenotomized tendons, adult male rats were divided into the following groups: G1, intact; G2, injured; G3, injured + LLL therapy (LLLT; 4 J/cm2 continuous); G4, injured + LLLT (4 J/cm2, 20 Hz); G5, injured; G6, injured + LLLT (4 J/cm2 continuous); and G7, injured + LLLT (4 J/cm2, 20 Hz until the 7th day and 2 kHz from 8 to 14 days). G2, G3, and G4 were euthanized 8 days after injury, and G5, G6, and G7 were euthanized on the 15th day. The quantification of hydroxyproline (HOPro) and non-collagenous protein (NCP), zymography for matrix metalloproteinase (MMP)-2 and MMP-9, and Western blotting (WB) for collagen types I and III were performed. HOPro levels showed a significant decrease in all groups (except G7) when compared with G1. The NCP level increased in all transected groups. WB for collagen type I showed an increase in G4 and G7. For collagen type III, G4 presented a higher value than G2. Zymography for MMP-2 indicated high values in G4 and G7. MMP-9 increased in both treatment groups euthanized at 8 days, especially in G4. Our results indicate that the pulsed LLLT improved the remodeling of the ECM during the healing process in tendons through activation of MMP-2 and stimulation of collagen synthesis.
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Metadata
Title
LLLT improves tendon healing through increase of MMP activity and collagen synthesis
Authors
Flávia Da Ré Guerra
Cristiano Pedrozo Vieira
Marcos Santos Almeida
Letícia Prado Oliveira
Andrea Aparecida de Aro
Edson Rosa Pimentel
Publication date
01-09-2013
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 5/2013
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-012-1236-7

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