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01-05-2013 | Original Article

Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro

Authors: Cristiane Aparecida Pereira, Anna Carolina Borges Pereira Costa, Claudia Moura Carreira, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge

Published in: Lasers in Medical Science | Issue 3/2013

Abstract

The purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using erythrosine (ER) and Rose Bengal (RB) photosensitizers and a blue light-emitting diode (LED) on the viability of Streptococcus mutans and Streptococcus sanguinis biofilms. Biofilms were grown in acrylic disks immersed in broth to production of biofilms, inoculated with microbial suspension (10⁶ cells/mL) and incubated for 48 h. After the formation of biofilms, the effects of the photosensitizers ER and RB at a concentration of 5 μM for 5 min and blue LED (455 ± 20 nm) for 180 s, photosensitizers alone and conjugated were evaluated. Next, the disks were placed in tubes with sterile physiological solution (0.9 % sodium chloride) and sonicated for to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in brain heart infusion agar which were then incubated for 48 h. Then the numbers colony-forming units per milliliter (CFU/mL; log₁₀) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by both photosensitizers. The reductions with RB and ER were, 0.62 and 0.52 log₁₀ CFU mL⁻¹ for S. mutans biofilms (p = 0.001), and 0.95 and 0.88 log₁₀ CFU mL⁻¹ for S. sanguinis biofilms (p = 0.001), respectively. The results showed that biofilms formed in vitro by S. mutans and S. sanguinis, were sensitive to PDI using a blue LED associated with photosensitizers ER or RB, indicating its use in the control of caries and periodontal diseases.

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Title: Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro
Authors: Cristiane Aparecida Pereira
Anna Carolina Borges Pereira Costa
Claudia Moura Carreira
Juliana Campos Junqueira
Antonio Olavo Cardoso Jorge
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 3/2013
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-012-1175-3

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Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro

Abstract

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Abstract

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