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01-09-2010 | Original Article

Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α

Authors: Flávia Mafra de Lima, Jan M. Bjordal, Regiane Albertini, Fábio V. Santos, Flavio Aimbire

Published in: Lasers in Medical Science | Issue 5/2010

Abstract

Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders. Bronchial smooth muscle (BSM) hyperreactivity is associated with increased Ca⁺² sensitivity and increased RhoA mRNA expression. In the current study, we investigated if LLLT could reduce BSM contraction force and RhoA mRNA expression in tumor necrosis factor-α (TNF-α)-induced BSM hyperreactivity. In the study, 112 male Wistar rats were divided randomly into 16 groups, and BSM was harvested and suspended in TNF-α baths for 6 and 24 h, respectively. Irradiation with LLLT was performed with a wavelength of 660 nm for 42 s with a dose of 1.3 J/cm². This LLLT dose was administered once in the 6-h group and twice in the 24-h group. LLLT significantly decreased contraction force in BSM at 6 h (TNF-α + LLLT: 11.65 ± 1.10 g/100 mg of tissue) (F = 3115) and at 24 h (TNF-α + LLLT: 14.15 ± 1.1 g/100 mg of tissue) (F = 3245, p < 0.05) after TNF-α, respectively, when compared to vehicle-bathed groups (control). LLLT also significantly decreased the expression of RhoA mRNA in BSM segments at 6 h (1.22 ± 0.20) (F = 2820, p < 0.05) and 24 h (2.13 ± 0.20) (F = 3324, p < 0.05) when compared to BSM segments incubated with TNF-α without LLLT irradiation. We conclude that LLLT administered with this protocol, reduces RhoA mRNA expression and BSM contraction force in TNF-α-induced BSM hyperreactivity.

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Title: Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α
Authors: Flávia Mafra de Lima
Jan M. Bjordal
Regiane Albertini
Fábio V. Santos
Flavio Aimbire
Publication date: 01-09-2010
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 5/2010
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-010-0766-0

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Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α

Abstract

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Abstract

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