Top

Published in:

01-09-2006 | Original Article

Effects of Nd:Yag laser irradiation on osteoblast cell cultures

Authors: Hacer Deniz Arisu, Emin Türköz, Oya Bala

Published in: Lasers in Medical Science | Issue 3/2006

Abstract

The application of Nd:YAG lasers in endodontics has been explored by many investigators for different purposes. The aim of this in vitro study is to evaluate the effects of Nd:YAG laser on osteoblast cell cultures. A 1,064 nm Nd:YAG laser was used to irradiate human osteoblast-like cells (Saos-2) in noncontact mode for 10 s with different energy settings. The settings varied were pulse energy (20–120 mJ), pulse repetition rate (10–30 Hz), and power output (0.2–3.6 W). He–Ne, the aiming beam of Nd:YAG laser, was used in noncontact mode for 10 s. After 7-, 14-, and 21-day incubation period, cell viability and proliferation were evaluated with methylthiazoletetrazolium (MTT) colorometric method. The comparison of average MTT values gave no statistically significant difference between 20 mJ, 10 Hz, He–Ne, and control (p<0.05). The differences between all other groups were significant (p<0.05). Increase in the pulse energy, pulse repetition rate, and power output has a negative effect on the cell viability and proliferation.

Yeh S, Jain K, Andreanas S (2005) Using a diode laser to uncover dental implants in second-stage surgery. Gen Dent 53:414–417PubMed

Cobb CM (2006) Lasers in periodontics: a review of the literature. J Periodontol 77:545–564PubMedCrossRef

Noguchi T, Sanaoka A, Fukuda M, Suzuki S, Aoki T (2005) Combined effects of Nd:YAG laser irradiation with local antibiotic application into periodontal pockets. J Int Acad Periodontol 7:8–15PubMed

Rodrigues LK, Dos Santos MN, Featherstone JD (2006) In situ mineral loss inhibition by CO2 laser and fluoride. J Dent Res 85:617–621PubMedCrossRef

Hossain M, Nakamura Y, Kimura Y, Yamada Y, Kawanaka T, Matsumoto K (2001) Effect of pulsed Nd:YAG laser irradiation on acid demineralization of enamel and dentin. J Clin Laser Med Surg 19:105–108PubMedCrossRef

Liu JF (2006) Effect of Nd:YAG laser pulpotomy on human primary molars. J Endod 32:404–407PubMedCrossRef

Stabholz A, Khayat A, Ravanshad SH, McCarthy DW, Neev J, Torabinejad M (1992) Effects of Nd:YAG laser on apical seal of teeth after apicoectomy and retrofill. J Endod 18:371–375PubMedCrossRef

Arisu HD, Bala O, Alimzhanova G, Turkoz E (2004) Assessment of morphological changes and permeability of apical dentin surfaces induced by Nd:YAG laser irradiation through retrograde cavity surfaces. J Contemp Dent Pract 5:102–113PubMed

Levy G (1992) Cleaning and shaping the root canal with a Nd:YAG laser beam: a comparative study. J Endod 18:123–127CrossRefPubMed

10.

Guthnecht N, Moritz A, Conrads G, Sievert T, Lampert F (1996) Bactericidal effect of the Nd:YAG laser in in vitro root canals. J Clin Laser Med Surg 14:77–80

11.

Bergamens L, Moisiadas P, Teughels W, Van Meerbeek B, Quirynen M, Lambrechts P (2006) Bactericidal effect of Nd:YAG laser irradiation on some endodontic pathogens ex vivo. Int Endod J 39:447–457

12.

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63PubMedCrossRef

13.

Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thompson D, bar-Shavit Z, Shull S, Mann K, Rodan GA (1987) Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. Cancer Res 47:4961–4966PubMed

14.

Murray E, Provvedini D, Curran D, Catherwood B, Sussman H, Manolagas S (1987) Characterization of human osteoblastic osteosarcoma cell line (SAOS-2) with high bone alkaline phosphatase activity. J Bone Miner Res 2:231–238PubMedCrossRef

15.

Tyas MJ (1977) A method for the in vitro toxicity testing of dental restorative materials. J Dent Res 56:1285–1290PubMed

16.

Huang FM, Tai KW, Chou MY, Chang YC (2002) Cytotoxicity of resin-, zinc oxide- eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 35:153–159PubMedCrossRef

17.

Vrouwenvelder WC, Groot CG, de Groot K (1993) Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy and stainless steel. J Biomed Mater Res 27:465–475PubMedCrossRef

18.

Kreisler M, Christoffers AB, Al-Haj H, Willerhausen B, D’Hoedt B (2002) Low level 809 nm diode laser induced in vitro stimulation of the proliferation of human gingival fibroblasts. Lasers Surg Med 30:365–369PubMedCrossRef

19.

Kreisler M, Daublander M, Willerhausen-Zönnchen B, D’Hoedt B (2001) Effects of diode laser irradiation on the survival rate of gingival fibroblast cell cultures. Lasers Surg Med 28:445–450PubMedCrossRef

Title: Effects of Nd:Yag laser irradiation on osteoblast cell cultures
Authors: Hacer Deniz Arisu
Emin Türköz
Oya Bala
Publication date: 01-09-2006
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 3/2006
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-006-0398-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of Nd:Yag laser irradiation on osteoblast cell cultures

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2006

Surgical treatment of erythroleukoplakia in lower lip with carbon dioxide laser radiation

Effect of Er,Cr:YSGG laser irradiation on eruption of rat mandibular incisor after disturbance of the enamel organ in the pulp

A review of the adverse effects of laser hair removal

Effects of the 1,320-nm Nd:YAG laser on transepidermal water loss, histological changes, and collagen remodeling in skin

Laser therapy and surgical treatment in transfusion-dependent patients with upper-gastrointestinal vascular ectasia

Holmium:YAG laser treatment of ureteral calculi: a 5-year experience