Skip to main content
Top
Published in: Lasers in Medical Science 4/2006

01-12-2006 | Original Article

The effects of infrared-830 nm laser on exercised osteopenic rats

Authors: Ana Claudia Muniz Renno, Fernanda Mendes de Moura, Nádia Slemer Andrade dos Santos, Renata Passarelli Tirico, Paulo Sérgio Bossini, Nivaldo Antonio Parizotto

Published in: Lasers in Medical Science | Issue 4/2006

Login to get access

Abstract

The aim of this study was to investigate the effects of low-level laser therapy (LLLT), 830 nm, on femora of exercised osteopenic rats. Sixty female rats were used, which were divided into six groups: sham-operated control, osteopenic control, sham-operated trained, osteopenic trained, sham-operated trained and irradiated, and osteopenic trained and irradiated. The exercise program and the laser irradiation were performed 48 h over an 8-week period. The exercise program was made in a container, filled with warm water, and consisted of jumps (four series, with ten jumps). The laser irradiation was performed with a Ga–Al–As laser, 830 nm, 100 W/cm2, 120 J/cm2. Femora were submitted to a physical and geometrical properties evaluation, a biomechanical test, and calcium and phosphorus evaluation. Exercised animals showed higher bone strength and physical properties values. However, the LLLT did not improve the stimulatory effects of the exercise on the osteopenic rats. The exercise program was able to increase femora strength and physical properties of osteopenic rats. However, concurrent treatments did not produce a more pronounced effect on femora.
Literature
1.
go back to reference No authors listed (1991) Consensus development conference: diagnosis, prophylaxis and treatment of osteoporosis. Osteoporos Int 1:114–117 No authors listed (1991) Consensus development conference: diagnosis, prophylaxis and treatment of osteoporosis. Osteoporos Int 1:114–117
2.
go back to reference Dempster DW (2003) Treatments of osteoporosis: how do they reduce the risk of fracture? Osteoporos Int 14(Suppl 7):S1 Dempster DW (2003) Treatments of osteoporosis: how do they reduce the risk of fracture? Osteoporos Int 14(Suppl 7):S1
3.
go back to reference Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:171–192CrossRef Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:171–192CrossRef
4.
go back to reference Bagi CM, Wilkie D, Georgelos K, Williams D, Bertolini D (1997) Morphological and structural characteristics of the proximal femur in human and rat. Bone 21:261–267PubMedCrossRef Bagi CM, Wilkie D, Georgelos K, Williams D, Bertolini D (1997) Morphological and structural characteristics of the proximal femur in human and rat. Bone 21:261–267PubMedCrossRef
5.
go back to reference Peng Z, Tuukkanen H, Zhang H, Jamsa T et al (1994) The mechanical strength of bone in different rat models of experimental osteoporosis. Bone 15:523–532PubMedCrossRef Peng Z, Tuukkanen H, Zhang H, Jamsa T et al (1994) The mechanical strength of bone in different rat models of experimental osteoporosis. Bone 15:523–532PubMedCrossRef
6.
go back to reference Nordsletten L et al (1994) The development of femoral osteopenia in ovariectomized rats is not reduced by high intensity treadmill training: a mechanical and densitometric study. Calcif Tissue Int 55(6):436–442PubMedCrossRef Nordsletten L et al (1994) The development of femoral osteopenia in ovariectomized rats is not reduced by high intensity treadmill training: a mechanical and densitometric study. Calcif Tissue Int 55(6):436–442PubMedCrossRef
7.
go back to reference Myburgh KH, Noakes TD, Roodt M, Hough FS (1989) Effect of exercise on the development of osteoporosis in adult rats. J Appl Physiol 66(1):14–19PubMed Myburgh KH, Noakes TD, Roodt M, Hough FS (1989) Effect of exercise on the development of osteoporosis in adult rats. J Appl Physiol 66(1):14–19PubMed
8.
go back to reference Donahue HJ, Mazzeo RS, Horvath SM (1988) Endurance training and bone loss in calcium-deficient and ovariectomized rats. Metabolism 37(8):741–744PubMedCrossRef Donahue HJ, Mazzeo RS, Horvath SM (1988) Endurance training and bone loss in calcium-deficient and ovariectomized rats. Metabolism 37(8):741–744PubMedCrossRef
9.
go back to reference Barengolts EI, Lathon PV, Curry DJ, Kukreja SC (1994) Effects of endurance exercise on bone histomorphometric parameters in intact and ovariectomized rats. Bone Miner 26(2):133–140PubMed Barengolts EI, Lathon PV, Curry DJ, Kukreja SC (1994) Effects of endurance exercise on bone histomorphometric parameters in intact and ovariectomized rats. Bone Miner 26(2):133–140PubMed
10.
go back to reference Luger EJ, Rochkind S, Wollman Y (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102PubMedCrossRef Luger EJ, Rochkind S, Wollman Y (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102PubMedCrossRef
11.
go back to reference David R, Nissan M, Cohen I (1996) Effect of low-power He–Ne laser on fracture healing in rats. Lasers Surg Med 19:458–464PubMedCrossRef David R, Nissan M, Cohen I (1996) Effect of low-power He–Ne laser on fracture healing in rats. Lasers Surg Med 19:458–464PubMedCrossRef
12.
go back to reference Mayayo E, Trelles MA (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7:36–45PubMed Mayayo E, Trelles MA (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7:36–45PubMed
13.
go back to reference Freitas IGF, Baranauskas V (2000) Lasers effects on osteogenesis. Appl Surf Sci 154:548–554CrossRef Freitas IGF, Baranauskas V (2000) Lasers effects on osteogenesis. Appl Surf Sci 154:548–554CrossRef
14.
go back to reference Renno ACM, Moura F, Bossini P, Parizotto N (2006) Effects of 830 nm laser, used in 2 doses, on biomechanical properties of osteopenic rats. Photomed Laser Surg 24:204–208 Renno ACM, Moura F, Bossini P, Parizotto N (2006) Effects of 830 nm laser, used in 2 doses, on biomechanical properties of osteopenic rats. Photomed Laser Surg 24:204–208
15.
go back to reference Ozawa Y, Shimizu N, Kariya G (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef Ozawa Y, Shimizu N, Kariya G (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef
16.
go back to reference Pinheiro ALB, Limeira F Jr, Gerbi M et al (2001) Biomodulatory effects of LLLT on bone regeneration. Laser Ther 13:73–79 Pinheiro ALB, Limeira F Jr, Gerbi M et al (2001) Biomodulatory effects of LLLT on bone regeneration. Laser Ther 13:73–79
17.
go back to reference Hornberger TA, Farrar RP (2004) Physiological hypertrophy of the FHL muscle following 8 weeks of progressive resistance exercise in the rat. Can J Appl Physiol 29(1):16–31PubMed Hornberger TA, Farrar RP (2004) Physiological hypertrophy of the FHL muscle following 8 weeks of progressive resistance exercise in the rat. Can J Appl Physiol 29(1):16–31PubMed
18.
go back to reference Silva A Jr, Pinheiro AL, Ramalho L, Nicolau R (2002) Computerized morphometric assessment of the effect of low-level laser therapy on bone repair: an experimental animal study. J Clin Laser Med Surg 20:83–87CrossRef Silva A Jr, Pinheiro AL, Ramalho L, Nicolau R (2002) Computerized morphometric assessment of the effect of low-level laser therapy on bone repair: an experimental animal study. J Clin Laser Med Surg 20:83–87CrossRef
19.
go back to reference Martin RB (1990) Effects of simulated weightlessness on bone properties in rats. J Biomech 23:1021–1029PubMedCrossRef Martin RB (1990) Effects of simulated weightlessness on bone properties in rats. J Biomech 23:1021–1029PubMedCrossRef
20.
go back to reference Wronski TJ, Dann LM, Scott KS, Cintron M (1989) Long-term effects of ovariectomy and aging on the rat skeleton. Calcif Tissue Int 45:360–366PubMed Wronski TJ, Dann LM, Scott KS, Cintron M (1989) Long-term effects of ovariectomy and aging on the rat skeleton. Calcif Tissue Int 45:360–366PubMed
21.
go back to reference Hietala EL (1993) The effect of ovariectomy on periosteal bone formation and bone resorption in adult rats. Bone Miner 20:57–65PubMedCrossRef Hietala EL (1993) The effect of ovariectomy on periosteal bone formation and bone resorption in adult rats. Bone Miner 20:57–65PubMedCrossRef
22.
go back to reference Li CY, Jee WS, Chen JL et al (2002) Estrogen and “exercise” have a synergistic effect in preventing bone loss in the lumbar vertebral and femoral neck of the ovariectomized rat. Calcif Tissue Int 72:42–49PubMedCrossRef Li CY, Jee WS, Chen JL et al (2002) Estrogen and “exercise” have a synergistic effect in preventing bone loss in the lumbar vertebral and femoral neck of the ovariectomized rat. Calcif Tissue Int 72:42–49PubMedCrossRef
23.
go back to reference Baxter GD (1997) Therapeutic lasers: theory and practice, 2nd edn. Churchill Livingstone, Edinburgh, pp 1–19 Baxter GD (1997) Therapeutic lasers: theory and practice, 2nd edn. Churchill Livingstone, Edinburgh, pp 1–19
24.
go back to reference Yamamoto C, Ogata N, Matsushima M, Takahashi K, Miyashiro M, Yamada H, Uyama M (1996) Expression of basic fibroblast growth factor and its receptor in the process of wound healing of rat retina after laser photocoagulation (in Japanese). Nippon Ganka Gakkai Zasshi 100:270–278PubMed Yamamoto C, Ogata N, Matsushima M, Takahashi K, Miyashiro M, Yamada H, Uyama M (1996) Expression of basic fibroblast growth factor and its receptor in the process of wound healing of rat retina after laser photocoagulation (in Japanese). Nippon Ganka Gakkai Zasshi 100:270–278PubMed
25.
go back to reference Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166PubMedCrossRef Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166PubMedCrossRef
26.
go back to reference Mester E, Mester A, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5(1):31–39PubMed Mester E, Mester A, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5(1):31–39PubMed
27.
go back to reference Renno ACM, Bossini PS, Mendes F, Parizotto N (2004) The effects of 830 nm laser, used in 2 doses, on femora of osteopenic rats. In: Proceedings 5th Congress World Association for Laser Therapy, Guaruja, Sao Paulo, Brazil, November 2004, pp 23–24 Renno ACM, Bossini PS, Mendes F, Parizotto N (2004) The effects of 830 nm laser, used in 2 doses, on femora of osteopenic rats. In: Proceedings 5th Congress World Association for Laser Therapy, Guaruja, Sao Paulo, Brazil, November 2004, pp 23–24
28.
go back to reference Werneck CE, Pinheiro AL, Pacheco MT, Soares CP et al (2005) Laser light is capable of inducing proliferation of carcinoma cells in culture: a spectroscopic in vitro study. Photomed Laser Surg 23:300–303PubMedCrossRef Werneck CE, Pinheiro AL, Pacheco MT, Soares CP et al (2005) Laser light is capable of inducing proliferation of carcinoma cells in culture: a spectroscopic in vitro study. Photomed Laser Surg 23:300–303PubMedCrossRef
29.
go back to reference Nicola RA, Jorgetti V, Rigau J, Zangaro R et al (2003) Effect of low-power GaAlAs laser (6607nbsp;nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94PubMedCrossRef Nicola RA, Jorgetti V, Rigau J, Zangaro R et al (2003) Effect of low-power GaAlAs laser (6607nbsp;nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94PubMedCrossRef
30.
go back to reference Kawasaki K, Shimizu N (2000) Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med 26:282–291PubMedCrossRef Kawasaki K, Shimizu N (2000) Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med 26:282–291PubMedCrossRef
Metadata
Title
The effects of infrared-830 nm laser on exercised osteopenic rats
Authors
Ana Claudia Muniz Renno
Fernanda Mendes de Moura
Nádia Slemer Andrade dos Santos
Renata Passarelli Tirico
Paulo Sérgio Bossini
Nivaldo Antonio Parizotto
Publication date
01-12-2006
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 4/2006
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-006-0396-8

Other articles of this Issue 4/2006

Lasers in Medical Science 4/2006 Go to the issue